Anti-Nucleophosmin 抗体 [FC82291] (ab10530)

製品の概要

  • 製品名
    Anti-Nucleophosmin antibody [FC82291]
    Nucleophosmin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [FC82291] to Nucleophosmin
  • 特異性
    Detects both the phosphorylated and the unphosphorylated B23 molecule.
  • アプリケーション
    適用あり: IP, IHC-P, ICC/IF, WB, ELISAmore details
  • 種交差性
    交差種: Mouse, Rat, Donkey, Hamster, Cow, Dog, Human, African green monkey
  • 免疫原

    Full length native protein (purified) (Rat).

  • エピトープ
    The epitope recognized by the antibody lies within the 68 amino acids at the C terminus of B23.
  • ポジティブ・コントロール
    • NIH3T3 whole cell lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 15mM Sodium Azide
    Constituents: 1% BSA, 0.01M PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    Protein G purified
  • ポリ/モノ
    モノクローナル
  • クローン名
    FC82291
  • アイソタイプ
    IgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab10530 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 33 kDa).
ELISA Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double-stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules. In concert with BRCA2, regulates centrosome duplication. Regulates centriole duplication: phosphorylation by PLK2 is able to trigger centriole replication. Negatively regulates the activation of EIF2AK2/PKR and suppresses apoptosis through inhibition of EIF2AK2/PKR autophosphorylation. Antagonizes the inhibitory effect of ATF5 on cell proliferation and relieves ATF5-induced G2/M blockade (PubMed:22528486).
  • 関連疾患
    A chromosomal aberration involving NPM1 is found in a form of non-Hodgkin lymphoma. Translocation t(2;5)(p23;q35) with ALK. The resulting chimeric NPM1-ALK protein homodimerize and the kinase becomes constitutively activated.
    A chromosomal aberration involving NPM1 is found in a form of acute promyelocytic leukemia. Translocation t(5;17)(q32;q11) with RARA.
    A chromosomal aberration involving NPM1 is a cause of myelodysplastic syndrome (MDS). Translocation t(3;5)(q25.1;q34) with MLF1.
    Defects in NPM1 are associated with acute myelogenous leukemia (AML). Mutations in exon 12 affecting the C-terminus of the protein are associated with an aberrant cytoplasmic location.
  • 配列類似性
    Belongs to the nucleoplasmin family.
  • 翻訳後修飾
    Acetylated at C-terminal lysine residues, thereby increasing affinity to histones.
    ADP-ribosylated.
    Phosphorylated at Ser-4 by PLK1 and PLK2. Phosphorylation at Ser-4 by PLK2 in S phase is required for centriole duplication and is sufficient to trigger centriole replication. Phosphorylation at Ser-4 by PLK1 takes place during mitosis. Phosphorylated by CDK2 at Ser-125 and Thr-199. Phosphorylation at Thr-199 may trigger initiation of centrosome duplication. Phosphorylated by CDK1 at Thr-199, Thr-219, Thr-234 and Thr-237 during cell mitosis. When these four sites are phosphorated, RNA-binding activity seem to be abolished. May be phosphorylated at Ser-70 by NEK2. The Thr-199 phosphorylated form has higher affinity for ROCK2. CDK6 triggers Thr-199 phosphorylation when complexed to Kaposi's sarcoma herpesvirus (KSHV) V-cyclin, leading to viral reactivation by reducing viral LANA levels.
    Sumoylated by ARF.
  • 細胞内局在
    Nucleus, nucleolus. Nucleus, nucleoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Generally nucleolar, but is translocated to the nucleoplasm in case of serum starvation or treatment with anticancer drugs. Has been found in the cytoplasm in patients with primary acute myelogenous leukemia (AML), but not with secondary AML. Can shuttle between cytoplasm and nucleus. Co- localizes with the methylated form of RPS10 in the granular component (GC) region of the nucleolus. Colocalized with nucleolin and APEX1 in nucleoli. Isoform 1 of NEK2 is required for its localization to the centrosome during mitosis.
  • Information by UniProt
  • 参照データベース
  • 別名
    • B23 antibody
    • MGC104254 antibody
    • NO38 antibody
    • NPM antibody
    • NPM_HUMAN antibody
    • NPM1 antibody
    • Nucleolar phosphoprotein B23 antibody
    • Nucleolar protein NO38 antibody
    • Nucleophosmin (nucleolar phosphoprotein B23 numatrin) antibody
    • Nucleophosmin antibody
    • nucleophosmin nucleoplasmin family member 1 antibody
    • Nucleophosmin/nucleoplasmin family member 1 antibody
    • Numatrin antibody
    • OTTHUMP00000161024 antibody
    • OTTHUMP00000161025 antibody
    • OTTHUMP00000223397 antibody
    • OTTHUMP00000223398 antibody
    see all

Anti-Nucleophosmin antibody [FC82291] 画像



  • Predicted band size : 33 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)

    This image is courtesy of an anonymous Abreview

    Western blot image of ab10530 staining whole cell lysate of U2OS cells.  The gel was blocked with 5% milk for 1 hour at 21°C. The primary antibody was diluted to 2 µg/ml and incubated  for 12 hours at 4°C.  A HRP conjugated goat anti-mouse antibody was used as the secondary.

    See Abreview

  • Anti-Nucleophosmin antibody [FC82291] (ab10530) at 0.2 µg/ml + 3T3 cell lysate

    Secondary
    Anti-Mouse IgG (Fab)

    Predicted band size : 33 kDa
  • All lanes : Anti-Nucleophosmin antibody [FC82291] (ab10530) at 0.5 µg/ml

    Lane 1 : HeLa cell lysate
    Lane 2 : JURKAT cell lysate
    Lane 3 : COS7 cell lysate
    Lane 4 : NIH-3T3 cell lysate
    Lane 5 : RAT2 cell lysate
    Lane 6 : CHO cell lysate
    Lane 7 : MDBK cell lysate
    Lane 8 : MDCK cell lysate

    Secondary
    Goat Anti-Mouse IgG-Peroxidase

    Predicted band size : 33 kDa

    Cell line lysate separated on SDS-PAGE, probed with ab10530 (0.5 μg/mL)

  • Immunocytochemistry/ Immunofluorescence analysis of HeLa cells staining Nucleophosmin using ab10530 (dilution 1/500). Cells were fixed and permeabilized with methanol followed by methanol:acetone. Developed using Goat Anti-Mouse IgG, Cy3™ conjugate and counterstained with DAPI (blue) to stain nuclei.

  • ab10530 staining Nucleophosmin in immortalized Human trabecular meshwork cells by Immunocytochemistry/ Immunofluorescence. Cells were PFA-fixed and permeabilized in 0.5% Triton X-100 prior to blocking in 3% BSA for 45 minutes at room temperature. The primary antibody was diluted 1/1000 in PBS/0.3% BSA and incubated with the sample for 2 hours. The secondary antibody was Cy3®-conjugated Donkey anti-Mouse polyclonal, diluted 1/1000.

    See Abreview

  • ab10530 at 1/250 staining human colon cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked with serum and incubated with the antibody for 30 minutes at 22°C. An HRP conjugated goat anti-mouse antibody was used as the secondary.

    See Abreview

  • ab10530 staining Nucleophosmin in Mouse cystic kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% serum for 30 minutes at 22°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/2500 in blocking buffer) for 30 minutes at 22°C. A HRP-conjugated Goat anti-mouse polyclonal (1/400) was used as the secondary antibody.

    See Abreview

Anti-Nucleophosmin antibody [FC82291] (ab10530) 使用論文

This product has been referenced in:
  • Huang M  et al. Autophagy mediates proteolysis of NPM1 and HEXIM1 and sensitivity to BET inhibition in AML cells. Oncotarget 7:74917-74930 (2016). WB ; Human . Read more (PubMed: 27732946) »
  • Goudarzi KM  et al. mTOR inhibitors blunt the p53 response to nucleolar stress by regulating RPL11 and MDM2 levels. Cancer Biol Ther 15:1499-514 (2014). Human . Read more (PubMed: 25482947) »

See all 20 Publications for this product

Product Wall

Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Pluripotent stem cell-derived retinal organoid)
Permeabilization
Yes - 0.1% Triton X100
Specification
Pluripotent stem cell-derived retinal organoid
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Methanol
Username

Abcam user community

Verified customer

投稿 Apr 11 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Human mast cell)
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
10 µg
Specification
Human mast cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Seong Hoon Kim

Verified customer

投稿 Jun 02 2016

Application
Western blot
Sample
Mouse Tissue lysate - whole (Cortex)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
40 µg
Specification
Cortex
Blocking step
Odyssey Blocking Buffer (PBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 45% · Temperature: 27°C
Username

Abcam user community

Verified customer

投稿 Oct 20 2015

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Neural stem cell)
Permeabilization
Yes - 0.2% TX100
Specification
Neural stem cell
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Apr 22 2015

Application
Western blot
Loading amount
100000 cells
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Human Cell lysate - whole cell (Brain neural stem cells)
Specification
Brain neural stem cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 19°C
Username

Abcam user community

Verified customer

投稿 Apr 08 2015

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (4-20%)
Sample
Mouse Tissue lysate - other (heart)
Specification
heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Sep 26 2014

Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - nuclear (U20s)
Specification
U20s
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Username

Abcam user community

Verified customer

投稿 Nov 13 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (U2OS)
Loading amount
20 µg
Specification
U2OS
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

投稿 Oct 08 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - nuclear (C17.2 mouse neural precursor cell line)
Loading amount
25 µg
Specification
C17.2 mouse neural precursor cell line
Gel Running Conditions
Reduced Denaturing (10% Novex Bis Tris)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Claire Thornton

Verified customer

投稿 Aug 30 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (U2OS)
Specification
U2OS
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% NP40/PBS
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Aug 27 2012

1-10 of 27 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録