Anti-Nucleophosmin 抗体 [FC82291] (ab10530)

製品の概要

  • 製品名Anti-Nucleophosmin antibody [FC82291]
    Nucleophosmin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [FC82291] to Nucleophosmin
  • 特異性Detects both the phosphorylated and the unphosphorylated B23 molecule.
  • アプリケーション適用あり: IP, IHC-P, ICC/IF, WB, ELISAmore details
  • 種交差性
    交差種: Mouse, Rat, Donkey, Hamster, Cow, Dog, Human, African Green Monkey
  • 免疫原

    Full length native protein (purified) (Rat).

  • エピトープThe epitope recognized by the antibody lies within the 68 amino acids at the C terminus of B23.
  • ポジティブ・コントロール
    • NIH3T3 whole cell lysate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 15mM Sodium Azide
    Constituents: 1% BSA, 0.01M PBS, pH 7.4
  • Concentration information loading...
  • 精製度Protein G purified
  • ポリ/モノモノクローナル
  • クローン名FC82291
  • アイソタイプIgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab10530 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 33 kDa).
ELISA Use at an assay dependent concentration.

ターゲット情報

  • 機能Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double-stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules. In concert with BRCA2, regulates centrosome duplication. Regulates centriole duplication: phosphorylation by PLK2 is able to trigger centriole replication. Negatively regulates the activation of EIF2AK2/PKR and suppresses apoptosis through inhibition of EIF2AK2/PKR autophosphorylation. Antagonizes the inhibitory effect of ATF5 on cell proliferation and relieves ATF5-induced G2/M blockade (PubMed:22528486).
  • 関連疾患A chromosomal aberration involving NPM1 is found in a form of non-Hodgkin lymphoma. Translocation t(2;5)(p23;q35) with ALK. The resulting chimeric NPM1-ALK protein homodimerize and the kinase becomes constitutively activated.
    A chromosomal aberration involving NPM1 is found in a form of acute promyelocytic leukemia. Translocation t(5;17)(q32;q11) with RARA.
    A chromosomal aberration involving NPM1 is a cause of myelodysplastic syndrome (MDS). Translocation t(3;5)(q25.1;q34) with MLF1.
    Defects in NPM1 are associated with acute myelogenous leukemia (AML). Mutations in exon 12 affecting the C-terminus of the protein are associated with an aberrant cytoplasmic location.
  • 配列類似性Belongs to the nucleoplasmin family.
  • 翻訳後修飾Acetylated at C-terminal lysine residues, thereby increasing affinity to histones.
    ADP-ribosylated.
    Phosphorylated at Ser-4 by PLK1 and PLK2. Phosphorylation at Ser-4 by PLK2 in S phase is required for centriole duplication and is sufficient to trigger centriole replication. Phosphorylation at Ser-4 by PLK1 takes place during mitosis. Phosphorylated by CDK2 at Ser-125 and Thr-199. Phosphorylation at Thr-199 may trigger initiation of centrosome duplication. Phosphorylated by CDK1 at Thr-199, Thr-219, Thr-234 and Thr-237 during cell mitosis. When these four sites are phosphorated, RNA-binding activity seem to be abolished. May be phosphorylated at Ser-70 by NEK2. The Thr-199 phosphorylated form has higher affinity for ROCK2. CDK6 triggers Thr-199 phosphorylation when complexed to Kaposi's sarcoma herpesvirus (KSHV) V-cyclin, leading to viral reactivation by reducing viral LANA levels.
    Sumoylated by ARF.
  • 細胞内局在Nucleus, nucleolus. Nucleus, nucleoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Generally nucleolar, but is translocated to the nucleoplasm in case of serum starvation or treatment with anticancer drugs. Has been found in the cytoplasm in patients with primary acute myelogenous leukemia (AML), but not with secondary AML. Can shuttle between cytoplasm and nucleus. Co- localizes with the methylated form of RPS10 in the granular component (GC) region of the nucleolus. Colocalized with nucleolin and APEX1 in nucleoli. Isoform 1 of NEK2 is required for its localization to the centrosome during mitosis.
  • Information by UniProt
  • 参照データベース
  • 別名
    • B23 antibody
    • MGC104254 antibody
    • NO38 antibody
    • NPM antibody
    • NPM_HUMAN antibody
    • NPM1 antibody
    • Nucleolar phosphoprotein B23 antibody
    • Nucleolar protein NO38 antibody
    • Nucleophosmin (nucleolar phosphoprotein B23 numatrin) antibody
    • Nucleophosmin antibody
    • nucleophosmin nucleoplasmin family member 1 antibody
    • Nucleophosmin/nucleoplasmin family member 1 antibody
    • Numatrin antibody
    • OTTHUMP00000161024 antibody
    • OTTHUMP00000161025 antibody
    • OTTHUMP00000223397 antibody
    • OTTHUMP00000223398 antibody
    see all

Anti-Nucleophosmin antibody [FC82291] 画像



  • Predicted band size : 33 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)

    This image is courtesy of an anonymous Abreview

    Western blot image of ab10530 staining whole cell lysate of U2OS cells.  The gel was blocked with 5% milk for 1 hour at 21°C. The primary antibody was diluted to 2 µg/ml and incubated  for 12 hours at 4°C.  A HRP conjugated goat anti-mouse antibody was used as the secondary.

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  • Immunocytochemistry/ Immunofluorescence analysis of HeLa cells staining Nucleophosmin using ab10530 (dilution 1/500). Cells were fixed and permeabilized with methanol followed by methanol:acetone. Developed using Goat Anti-Mouse IgG, Cy3™ conjugate and counterstained with DAPI (blue) to stain nuclei.

  • ab10530 at 1/250 staining human colon cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked with serum and incubated with the antibody for 30 minutes at 22°C. An HRP conjugated goat anti-mouse antibody was used as the secondary.

    See Abreview

  • Anti-Nucleophosmin antibody [FC82291] (ab10530) at 0.2 µg/ml + 3T3 cell lysate

    Secondary
    Anti-Mouse IgG (Fab)

    Predicted band size : 33 kDa
  • ab10530 staining Nucleophosmin in immortalized Human trabecular meshwork cells by Immunocytochemistry/ Immunofluorescence. Cells were PFA-fixed and permeabilized in 0.5% Triton X-100 prior to blocking in 3% BSA for 45 minutes at room temperature. The primary antibody was diluted 1/1000 in PBS/0.3% BSA and incubated with the sample for 2 hours. The secondary antibody was Cy3®-conjugated Donkey anti-Mouse polyclonal, diluted 1/1000.

    See Abreview

  • All lanes : Anti-Nucleophosmin antibody [FC82291] (ab10530) at 0.5 µg/ml

    Lane 1 : HeLa cell lysate
    Lane 2 : JURKAT cell lysate
    Lane 3 : COS7 cell lysate
    Lane 4 : NIH-3T3 cell lysate
    Lane 5 : RAT2 cell lysate
    Lane 6 : CHO cell lysate
    Lane 7 : MDBK cell lysate
    Lane 8 : MDCK cell lysate

    Secondary
    Goat Anti-Mouse IgG-Peroxidase

    Predicted band size : 33 kDa

    Cell line lysate separated on SDS-PAGE, probed with ab10530 (0.5 μg/mL)

Anti-Nucleophosmin antibody [FC82291] (ab10530) 使用論文

This product has been referenced in:
  • Taha MS  et al. Subcellular fractionation and localization studies reveal a direct interaction of the fragile X mental retardation protein (FMRP) with nucleolin. PLoS One 9:e91465 (2014). ICC/IF ; Human . Read more (PubMed: 24658146) »
  • Ponti D  et al. The transcription factor EGR1 localizes to the nucleolus and is linked to suppression of ribosomal precursor synthesis. PLoS One 9:e96037 (2014). WB ; Human . Read more (PubMed: 24787739) »

See all 19 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Human mast cell)
Gel Running Conditions Non-reduced Non-Denaturing (Native)
Loading amount 10 µg
Specification Human mast cell
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Mr. Seong Hoon Kim

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投稿 Jun 02 2016

Application Western blot
Sample Mouse Tissue lysate - whole (Cortex)
Gel Running Conditions Reduced Denaturing (10)
Loading amount 40 µg
Specification Cortex
Blocking step Odyssey Blocking Buffer (PBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 45% · Temperature: 27°C
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投稿 Oct 20 2015

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Neural stem cell)
Permeabilization Yes - 0.2% TX100
Specification Neural stem cell
Fixative Formaldehyde
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投稿 Apr 22 2015

Application Western blot
Loading amount 100000 cells
Gel Running Conditions Reduced Denaturing (10%)
Sample Human Cell lysate - whole cell (Brain neural stem cells)
Specification Brain neural stem cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 19°C
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投稿 Apr 08 2015

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (4-20%)
Sample Mouse Tissue lysate - other (heart)
Specification heart
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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投稿 Sep 26 2014

Application Western blot
Loading amount 40 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - nuclear (U20s)
Specification U20s
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
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投稿 Nov 13 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (U2OS)
Loading amount 20 µg
Specification U2OS
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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投稿 Oct 08 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - nuclear (C17.2 mouse neural precursor cell line)
Loading amount 25 µg
Specification C17.2 mouse neural precursor cell line
Gel Running Conditions Reduced Denaturing (10% Novex Bis Tris)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Claire Thornton

Verified customer

投稿 Aug 30 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (U2OS)
Specification U2OS
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% NP40/PBS
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
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投稿 Aug 27 2012

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