Anti-Nucleophosmin 抗体 [EP1848Y] (ab52644)

製品の概要

  • 製品名
    Anti-Nucleophosmin antibody [EP1848Y]
    Nucleophosmin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1848Y] to Nucleophosmin
  • アプリケーション
    適用あり: WB, IP, Flow Cyt, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to residues from the N terminus of human Nucleophosmin.

  • ポジティブ・コントロール
    • WB: HeLa cell lysate. IHC-P: Human glioma, breast carcinoma and cervical carcinoma tissues.
  • 特記事項

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 精製度
    Tissue culture supernatant
  • ポリ/モノ
    モノクローナル
  • クローン名
    EP1848Y
  • アイソタイプ
    IgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab52644 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/200000. Detects a band of approximately 35,38 kDa (predicted molecular weight: 33 kDa).
IP 1/60.
Flow Cyt 1/40.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double-stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules. In concert with BRCA2, regulates centrosome duplication. Regulates centriole duplication: phosphorylation by PLK2 is able to trigger centriole replication. Negatively regulates the activation of EIF2AK2/PKR and suppresses apoptosis through inhibition of EIF2AK2/PKR autophosphorylation. Antagonizes the inhibitory effect of ATF5 on cell proliferation and relieves ATF5-induced G2/M blockade (PubMed:22528486).
  • 関連疾患
    A chromosomal aberration involving NPM1 is found in a form of non-Hodgkin lymphoma. Translocation t(2;5)(p23;q35) with ALK. The resulting chimeric NPM1-ALK protein homodimerize and the kinase becomes constitutively activated.
    A chromosomal aberration involving NPM1 is found in a form of acute promyelocytic leukemia. Translocation t(5;17)(q32;q11) with RARA.
    A chromosomal aberration involving NPM1 is a cause of myelodysplastic syndrome (MDS). Translocation t(3;5)(q25.1;q34) with MLF1.
    Defects in NPM1 are associated with acute myelogenous leukemia (AML). Mutations in exon 12 affecting the C-terminus of the protein are associated with an aberrant cytoplasmic location.
  • 配列類似性
    Belongs to the nucleoplasmin family.
  • 翻訳後修飾
    Acetylated at C-terminal lysine residues, thereby increasing affinity to histones.
    ADP-ribosylated.
    Phosphorylated at Ser-4 by PLK1 and PLK2. Phosphorylation at Ser-4 by PLK2 in S phase is required for centriole duplication and is sufficient to trigger centriole replication. Phosphorylation at Ser-4 by PLK1 takes place during mitosis. Phosphorylated by CDK2 at Ser-125 and Thr-199. Phosphorylation at Thr-199 may trigger initiation of centrosome duplication. Phosphorylated by CDK1 at Thr-199, Thr-219, Thr-234 and Thr-237 during cell mitosis. When these four sites are phosphorated, RNA-binding activity seem to be abolished. May be phosphorylated at Ser-70 by NEK2. The Thr-199 phosphorylated form has higher affinity for ROCK2. CDK6 triggers Thr-199 phosphorylation when complexed to Kaposi's sarcoma herpesvirus (KSHV) V-cyclin, leading to viral reactivation by reducing viral LANA levels.
    Sumoylated by ARF.
  • 細胞内局在
    Nucleus, nucleolus. Nucleus, nucleoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Generally nucleolar, but is translocated to the nucleoplasm in case of serum starvation or treatment with anticancer drugs. Has been found in the cytoplasm in patients with primary acute myelogenous leukemia (AML), but not with secondary AML. Can shuttle between cytoplasm and nucleus. Co- localizes with the methylated form of RPS10 in the granular component (GC) region of the nucleolus. Colocalized with nucleolin and APEX1 in nucleoli. Isoform 1 of NEK2 is required for its localization to the centrosome during mitosis.
  • Information by UniProt
  • 参照データベース
  • 別名
    • B23 antibody
    • MGC104254 antibody
    • NO38 antibody
    • NPM antibody
    • NPM_HUMAN antibody
    • NPM1 antibody
    • Nucleolar phosphoprotein B23 antibody
    • Nucleolar protein NO38 antibody
    • Nucleophosmin (nucleolar phosphoprotein B23 numatrin) antibody
    • Nucleophosmin antibody
    • nucleophosmin nucleoplasmin family member 1 antibody
    • Nucleophosmin/nucleoplasmin family member 1 antibody
    • Numatrin antibody
    • OTTHUMP00000161024 antibody
    • OTTHUMP00000161025 antibody
    • OTTHUMP00000223397 antibody
    • OTTHUMP00000223398 antibody
    see all

Anti-Nucleophosmin antibody [EP1848Y] 画像

  • Anti-Nucleophosmin antibody [EP1848Y] (ab52644) at 1/200000 dilution + Hela cell lysate at 10 µg

    Secondary
    Goat anti-rabbit HRP labeled at 1/2000 dilution

    Predicted band size : 33 kDa
    Observed band size : 35 & 38 kDa (why is the actual band size different from the predicted?)
  • ab52644 showing positive staining in Breast carcinoma tissue.

  • ab52644 showing positive staining in Cervical carcinoma tissue.

  • ab52644 showing positive staining in Glioma tissue.

Anti-Nucleophosmin antibody [EP1848Y] (ab52644) 使用論文

This product has been referenced in:
  • Marsh LA  et al. BASP1 interacts with oestrogen receptor a and modifies the tamoxifen response. Cell Death Dis 8:e2771 (2017). Human . Read more (PubMed: 28492543) »
  • Chang CY  et al. Up-regulation of ribosome biogenesis by MIR196A2 genetic variation promotes endometriosis development and progression. Oncotarget 7:76713-76725 (2016). IHC ; Human . Read more (PubMed: 27741504) »

See all 7 Publications for this product

Product Wall

Application
Immunoprecipitation
Immuno-precipitation step
Protein A/G
Sample
Human Cell lysate - whole cell (HEK293 cells)
Specification
HEK293 cells
Total protein in input
500 µg
Username

Abcam user community

Verified customer

投稿 Aug 28 2014

Thank you for contacting us.

There is no need for special secondary antibody. Any normal anti rabbit secondary ab would work.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice ...

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Thank you for contacting us.

We normally use Nupage 4-12% Bis Tris gel for western blot.

The dilution is correct. It is indeed 1/200,000 - this is because it is a rabbit monoclonal antibody. These happen to be more specific and can wo...

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Thank you for contacting us.

The peptide maps between amino acids 1 - 30 of human NPM (UniProt: P06748).

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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