製品の概要

  • 製品名Anti-Nucleolin antibody
    Nucleolin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Nucleolin
  • アプリケーション適用あり: WB, IP, ICC/IF, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Cynomolgus Monkey, Orangutan
  • 免疫原

    Synthetic peptide:

    VKLAKAGKNQGDPKKMA

    conjugated to KLH, corresponding to N terminal amino acids 2-17 of Human Nucleolin with a C-terminal added cysteine.

  • ポジティブ・コントロール
    • Whole extract of mouse NIH3T3 cells or rat PC12 cells, or human HeLa cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab50279 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 77 kDa.
IP Use a concentration of 1 - 2 µg/ml.

1-2 µg/ml immunoprecipitates Nucleolin from 250 ug RIPA lysate of human HeLa cells.

ICC/IF Use a concentration of 10 - 20 µg/ml.
Flow Cyt Use at an assay dependent concentration. PubMed: 20814900

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.
  • 配列類似性Contains 4 RRM (RNA recognition motif) domains.
  • 翻訳後修飾Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group.
  • 細胞内局在Nucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Information by UniProt
  • 参照データベース
  • 別名
    • C23 antibody
    • FLJ45706 antibody
    • MS1116 antibody
    • NCL antibody
    • Nucl antibody
    • NUCL_HUMAN antibody
    • Nucleolin antibody
    • Protein C23 antibody
    see all

Anti-Nucleolin antibody 画像

  • Indirect Immunofluorescence

    Human HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 10 µg/mL Anti-Nucleolin (ab50279). The antibody was developed with Goat Anti-Rabbit IgG, FITC conjugate.

Anti-Nucleolin antibody (ab50279) 使用論文

This product has been referenced in:
  • Bender BJ  et al. Dynamic and nucleolin-dependent localization of human cytomegalovirus UL84 to the periphery of viral replication compartments and nucleoli. J Virol 88:11738-47 (2014). Read more (PubMed: 25078694) »
  • Peddigari S  et al. hnRNPL and nucleolin bind LINE-1 RNA and function as host factors to modulate retrotransposition. Nucleic Acids Res 41:575-85 (2013). WB ; Human . Read more (PubMed: 23161687) »

See all 9 Publications for this product

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