Anti-Nucleolin 抗体 [364-5] (ab136649)
Key features and details
- Mouse monoclonal [364-5] to Nucleolin
- Suitable for: ICC/IF, IHC-P, WB, IP, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Related conjugates and formulations
製品の概要
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製品名
Anti-Nucleolin antibody [364-5]
Nucleolin 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [364-5] to Nucleolin -
由来種
Mouse -
アプリケーション
適用あり: ICC/IF, IHC-P, WB, IP, Flow Cytmore details -
種交差性
交差種: Human
非交差種: Mouse, Rat, Cow -
免疫原
Tissue, cells or virus. This information is considered to be commercially sensitive.
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ポジティブ・コントロール
- HeLa and HDFn cells; HepG2, HDFn and SH-SY5Y whole cell lysates.
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.88% Sodium chloride, 0.36% HEPES -
Concentration information loading...
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特記事項(精製)
ab136649 is purified by biochemical fractionation. Near homogeneity as judged by SDS-PAGE. -
ポリ/モノ
モノクローナル -
クローン名
364-5 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab136649の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF | (2) |
Use a concentration of 1 µg/ml.
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IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 76 kDa.
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IP |
Use a concentration of 1 µg/ml.
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Flow Cyt |
Use a concentration of 1 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
特記事項 |
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ICC/IF
Use a concentration of 1 µg/ml. |
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 76 kDa. |
IP
Use a concentration of 1 µg/ml. |
Flow Cyt
Use a concentration of 1 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats. -
配列類似性
Contains 4 RRM (RNA recognition motif) domains. -
翻訳後修飾
Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group. -
細胞内局在
Nucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. - Information by UniProt
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参照データベース
- Entrez Gene: 4691 Human
- Omim: 164035 Human
- SwissProt: P19338 Human
- Unigene: 79110 Human
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別名
- C23 antibody
- FLJ45706 antibody
- MS1116 antibody
see all
画像
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ab136649 staining Nucleolin in human breast cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 5% Triton X-100 in PBS and blocked with 5% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/1000) for 1 hour. An undiluted Alexa Fluor® 546-conjugated anti-mouse IgG polyclonal was used as the secondary antibody.
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All lanes : Anti-Nucleolin antibody [364-5] (ab136649) at 1 µg/ml
Lane 1 : HepG2 whole cell lysate at 20 µg
Lane 2 : HDFn whole cell lysate at 25 µg
Lane 3 : SH-SY5Y whole cell lysate at 20 µg
Secondary
All lanes : Goat polyclonal to Mouse IgG - AP at 1/3000 dilution
Predicted band size: 76 kDa -
Immunocytochemical analysis of HeLa cells (paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15 min)) labelling Nucleolin with ab136649 at 1µg/ml (red) and anti-GAPDH (ab110305) in green, as a counter stain.
Secondary: Alexa 594 goat anti-mouse IgG1 at a 1/1000 dilution. -
Immunocytochemical analysis of HDFn cells (paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15 min)) labelling Nucleolin with ab136649 at 1µg/ml (red) and anti-GAPDH (ab110305) in green, as a counter stain. Secondary: Alexa 594 goat anti-mouse IgG1 at a 1/1000 dilution.
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Detection of Nucleolin by Immunoprecipitation.
Nucleolin in HepG2 cell lysate (1 mg) was immunoprecipitated using 1 µg ab136649 antibody. The gel was stained with Coomassie brilliant blue G. -
Flow Cytometric analysis of HeLa cells (fixed and permeabilized with methanol) labelling Nucleolin with ab136649 (blue) at 1µg/ml or an equal amount of an isotype control antibody (red).
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IHC image of nucleolin staining in human liver HCC formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab136649, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (11)
ab136649 は 11 報の論文で使用されています。
- Champagne A et al. A transcriptional biosensor to monitor single cancer cell therapeutic responses by bioluminescence microscopy. Theranostics 12:474-492 (2022). PubMed: 34976196
- Li F et al. The Atr-Chek1 pathway inhibits axon regeneration in response to Piezo-dependent mechanosensation. Nat Commun 12:3845 (2021). PubMed: 34158506
- Ovejero S et al. The Alkylating Agent Methyl Methanesulfonate Triggers Lipid Alterations at the Inner Nuclear Membrane That Are Independent from Its DNA-Damaging Ability. Int J Mol Sci 22:N/A (2021). PubMed: 34299079
- Nihei Y et al. Poly-glycine-alanine exacerbates C9orf72 repeat expansion-mediated DNA damage via sequestration of phosphorylated ATM and loss of nuclear hnRNPA3. Acta Neuropathol 139:99-118 (2020). PubMed: 31642962
- Dubois ML et al. UBB pseudogene 4 encodes functional ubiquitin variants. Nat Commun 11:1306 (2020). PubMed: 32161257