Anti-Nucleobindin 1 抗体 (ab55768)

製品の概要

  • 製品名Anti-Nucleobindin 1 antibody
    Nucleobindin 1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to Nucleobindin 1
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length protein, corresponding to amino acids 1-297 of Human Nucleobindin 1

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein G purified
  • ポリ/モノモノクローナル
  • アイソタイプIgG1
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab55768 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IHC-P
  • 追加情報WB: Use at a concentration of 1-5 µg/ml.
    IHC-P: Use at a concentration of 3 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Major calcium-binding protein of the Golgi. May have a role in calcium homeostasis.
    • 組織特異性Expressed both in fetal and adult heart, lung, liver, kidney and brain, and in adult skeletal muscle, placenta and pancreas.
    • 配列類似性Belongs to the nucleobindin family.
      Contains 2 EF-hand domains.
    • ドメインThe EF-hand domains are unfolded in the absence of Ca(2+) and fold upon Ca(2+) addition.
    • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 細胞内局在Golgi apparatus > cis-Golgi network membrane. Cytoplasm. A small fraction of the protein may be cytoplasmic.
    • Information by UniProt
    • 参照データベース
    • 別名
      • CALNUC antibody
      • FLJ40471 antibody
      • NUC antibody
      • NUCB 1 antibody
      • NUCB1 antibody
      • NUCB1_HUMAN antibody
      • Nucleobindin 1 antibody
      • Nucleobindin-1 antibody
      see all

    Anti-Nucleobindin 1 antibody 画像

    • Nucleobindin 1 antibody (ab55768) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human spleen tissue.


    • Predicted band size : 54 kDa
      Nucleobindin 1 antibody (ab55768) at 1ug/lane + HepG2 cell lysate at 25ug/lane.

    Anti-Nucleobindin 1 antibody (ab55768) 使用論文

    ab55768 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample Rat Tissue sections (Brain slices)
    Specification Brain slices
    Fixative 4% paraformaldehyde + 0.2% picric acid
    Antigen retrieval step None
    Permeabilization Yes - Triton X-100
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
    Username

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    投稿 Aug 24 2012

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    The expiration date refers to the date in which the discount code for the free antibody should be used by. As you are yet to submit the Abreviews I can issue 2 more codes as an exception. These will be valid for ...

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    DISCOUNT CODE (ab93373): XXXXXXXXXX
    DISCOUNT CODE (ab55768): XXXXXXXXXX

    Expiration date: 22-05-2012

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (MDA-MB-231 breast cancer cell line)
    Loading amount 40 µg
    Specification MDA-MB-231 breast cancer cell line
    Gel Running Conditions Reduced Denaturing (gel 12 %)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

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    Verified customer

    投稿 Oct 15 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"