Anti-Nucleobindin 1 抗体 (ab23360)

製品の概要

  • 製品名Anti-Nucleobindin 1 antibody
    Nucleobindin 1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Nucleobindin 1
  • 特異性ab23360 recognises Nucleobindin 1
  • アプリケーション適用あり: WB, ICC, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide: PAAHPEGQLK FHPDTDDVPV PAPAGDQKEV DTSEKKLLER LPEVEVPQHL conjugated to KLH, corresponding to a region within C terminal amino acids 413-462 of Human Nucleobindin 1

  • ポジティブ・コントロール
    • HeLa cells, HepG2

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab23360 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration. Predicted molecular weight: 54 kDa.
ICC Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

ターゲット情報

  • 機能Major calcium-binding protein of the Golgi. May have a role in calcium homeostasis.
  • 組織特異性Expressed both in fetal and adult heart, lung, liver, kidney and brain, and in adult skeletal muscle, placenta and pancreas.
  • 配列類似性Belongs to the nucleobindin family.
    Contains 2 EF-hand domains.
  • ドメインThe EF-hand domains are unfolded in the absence of Ca(2+) and fold upon Ca(2+) addition.
  • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在Golgi apparatus > cis-Golgi network membrane. Cytoplasm. A small fraction of the protein may be cytoplasmic.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CALNUC antibody
    • FLJ40471 antibody
    • NUC antibody
    • NUCB 1 antibody
    • NUCB1 antibody
    • NUCB1_HUMAN antibody
    • Nucleobindin 1 antibody
    • Nucleobindin-1 antibody
    see all

Anti-Nucleobindin 1 antibody 画像

  • Golgi of HeLa cells are detected with ab23360 (green). Nuclei (red) were stained with DAPI.
  • Anti-Nucleobindin 1 antibody (ab23360) at 1 µg/ml + HepG2 lysates

    Predicted band size : 54 kDa

Anti-Nucleobindin 1 antibody (ab23360) 使用論文

ab23360 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (MDA-MB-231 breast cancer cell line)
Specification MDA-MB-231 breast cancer cell line
Fixative Paraformaldehyde
Permeabilization Yes - Triton 0.03%
Blocking step Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 25°C
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投稿 Oct 09 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"