Anti-Nuclear Receptor Corepressor NCoR 抗体 - ChIP Grade (ab24552)

製品の概要

  • 製品名
    Anti-Nuclear Receptor Corepressor NCoR antibody - ChIP Grade
    Nuclear Receptor Corepressor NCoR 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Nuclear Receptor Corepressor NCoR - ChIP Grade
  • 特異性
    This antibody is specific to NCoR1,variant 1, Genbank Accession number AF044209, SwissProt ID O75376.
  • アプリケーション
    適用あり: ChIP, IP, WB, ICC/IFmore details
  • 種交差性
    交差種: Human, Monkey
  • 免疫原

    Recombinant fragment, corresponding to amino acids 789-957 of Human Nuclear Receptor Corepressor NCoR

  • ポジティブ・コントロール
    • HeLa nuclear lysate

アプリケーション

Our Abpromise guarantee covers the use of ab24552 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ChIP Use at an assay dependent dilution.
IP Use at an assay dependent dilution. Use 2-3ul per IP (See references for protocol details).
WB 1/1000. Detects a band of approximately 270 kDa (predicted molecular weight: 270 kDa).
ICC/IF Use at an assay dependent dilution.

ターゲット情報

  • 機能
    Mediates transcriptional repression by certain nuclear receptors. Part of a complex which promotes histone deacetylation and the formation of repressive chromatin structures which may impede the access of basal transcription factors.
  • 配列類似性
    Belongs to the N-CoR nuclear receptor corepressors family.
    Contains 2 SANT domains.
  • ドメイン
    The N-terminal region contains three independent domains that are capable of mediating transcriptional repression (RD1, RD2 and RD3).
    The C-terminal region contains two separate nuclear receptor-interacting domains (ID1 and ID2), each of which contains a conserved sequence referred to as the CORNR box. This motif is necessary and sufficient for binding to unligated nuclear hormone receptors, while sequences flanking the CORNR box determine the precise nuclear hormone receptor specificity.
  • 翻訳後修飾
    Ubiquitinated; mediated by SIAH2 and leading to its subsequent proteasomal degradation.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • hN CoR antibody
    • hNCoR antibody
    • KIAA1047 antibody
    • N CoR antibody
    • N Cor/SMRT corepressor Rip13 antibody
    • N CoR1 antibody
    • N-CoR antibody
    • N-CoR1 antibody
    • NCOR 1 antibody
    • NCoR antibody
    • Ncor1 antibody
    • NCOR1_HUMAN antibody
    • Nuclear receptor co repressor 1 antibody
    • Nuclear receptor corepressor 1 antibody
    • Retinoid X receptor interacting protein 13 antibody
    • RIP13 antibody
    • Rxrip13 antibody
    • thyroid hormone and retinoic acid receptor associated corepressor 1 antibody
    • TRAC 1 antibody
    • TRAC1 antibody
    see all

Anti-Nuclear Receptor Corepressor NCoR antibody - ChIP Grade 画像

  • Anti-Nuclear Receptor Corepressor NCoR antibody - ChIP Grade (ab24552) at 1/1000 dilution + HeLa nuclear extract

    Predicted band size : 270 kDa
    Observed band size : 270 kDa

Anti-Nuclear Receptor Corepressor NCoR antibody - ChIP Grade (ab24552) 使用論文

This product has been referenced in:
  • Chakraborty K  et al. The mito-DAMP cardiolipin blocks IL-10 production causing persistent inflammation during bacterial pneumonia. Nat Commun 8:13944 (2017). Read more (PubMed: 28074841) »
  • Viswakarma N  et al. Transcriptional regulation of mixed lineage kinase 3 by estrogen and its implication in ER-positive breast cancer pathogenesis. Oncotarget 8:33172-33184 (2017). ChIP . Read more (PubMed: 28388540) »

See all 26 Publications for this product

Product Wall

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing
Sample
Rat Tissue lysate - whole (liver)
Specification
liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Apr 07 2014

Application
ChIP
Detection step
Semiquantitative PCR
Sample
Human Cell lysate - nuclear (MCF7)
Specification
MCF7
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: FORMALDEHYDE 10%
Username

Abcam user community

Verified customer

投稿 Dec 10 2013

Thank you for your reply.

I am sorry the results you have observed have not improved with ab24552. From looking at the results you have shared you appear to be getting very similar results, with ab24552 showing a much clearer band at ˜250 kDa ...

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Thank you for contacting Abcam earlier today.
As we discussed, I am sending you new aliquots of both ab24552 and ab26721 and your new order number is ***********.
Below are a few possible alternatives if the replacements are not successful: <...

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Vielen Dank für Ihre Anfrage - und für Ihre Geduld! Bitte entschuldigen Sie, dass die Antwort so lange auf sich warten ließ. Ich hatte das Labor, das den NCoR- Antikörper ab24552 herstellt, kontaktiert und musste sehr lange auf eine zufriedenstellen...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (vero)
Loading amount
20000 cells
Specification
vero
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 minute(s) · Concentration: 0.1% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Jun 23 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - nuclear (MCF-7 Breast Cancer cells)
Loading amount
25 µg
Specification
MCF-7 Breast Cancer cells
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Dr. Corina Marx

Verified customer

投稿 Jul 20 2007

I have just received information from the source of the antibody saying "We normally use 2-3 ul of the N-CoR antibody for IP. We normally use HeLa nuclear extracts for IP, although it also worked for whole cell extracts from HeLa or 293T. I attach a co...

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Thank you for your enquiry. We qualify our "ChIP grade" antibodies in three ways: Through our; 1. In our in-house ChIP testing program, 2. In a scientific publication or 3. by a customer review. On this occasion this antibody was sourced fr...

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Thank you for your enquiry. We qualify our antibodies as "ChIP grade" status in three ways 1. in our in-house ChIP testing program, 2. in a scientific publication and 3. by a customer (Abreview). Rabbit polyclonal to NCoR2, ab24551 was qualifie...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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