Anti-Nuclear Pore Complex Proteins 抗体 [Mab414] (ab24609)
Key features and details
- Mouse monoclonal [Mab414] to Nuclear Pore Complex Proteins
- Suitable for: ICC/IF
- Reacts with: Mouse, Human, Saccharomyces cerevisiae
- Isotype: IgG1
製品の概要
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製品名
Anti-Nuclear Pore Complex Proteins antibody [Mab414] -
製品の詳細
Mouse monoclonal [Mab414] to Nuclear Pore Complex Proteins -
由来種
Mouse -
アプリケーション
適用あり: ICC/IFmore details -
種交差性
交差種: Mouse, Human, Saccharomyces cerevisiae
交差が予測される動物種: Vertebrata -
免疫原
Full length native protein (purified) corresponding to Human Nuclear Pore Complex Proteins. Nuclear Pore Complex Proteins
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ポジティブ・コントロール
- Raw, HEK 293 cell lysate (see Abreview), rat liver lysate (see Aris reference)
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特記事項
This is a reliable general purpose monoclonal antibody which recognizes a related family of NPC proteins. This antibody is ideal for studying the morphology and composition of the nucleus and nuclear envelope. It is also useful in studying changes in the nuclear structure during mitosis and meiosis.
This product was changed from ascites to tissue culture supernatant on 20/05/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.03% Thimerosal (merthiolate)
Constituent: PBS -
Concentration information loading...
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精製度
Affinity purified -
一次抗体 備考
This is a reliable general purpose monoclonal antibody which recognizes a related family of NPC proteins. This antibody is ideal for studying the morphology and composition of the nucleus and nuclear envelope. It is also useful in studying changes in the nuclear structure during mitosis and meiosis. -
ポリ/モノ
モノクローナル -
クローン名
Mab414 -
アイソタイプ
IgG1 -
研究分野
関連製品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab24609の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF | (8) |
Use at an assay dependent concentration.
Fix cells with 4% paraformaldehyde in NWB (200 mM sucrose, 15 mM Hepes, pH 7.4, 50 mM NaCl, 2.5 mM MgCl2, and 1 mM DTT). Permeabilise with 0.1% NP-40 or 0.1% Triton X-100 in PBS for 2 min. (see Lopez-Soler reference); different customer have used this antibody at different dilutions for ICC/IF (see images below). We recommend that optimal working dilutions are determined by each customer. |
特記事項 |
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ICC/IF
Use at an assay dependent concentration. Fix cells with 4% paraformaldehyde in NWB (200 mM sucrose, 15 mM Hepes, pH 7.4, 50 mM NaCl, 2.5 mM MgCl2, and 1 mM DTT). Permeabilise with 0.1% NP-40 or 0.1% Triton X-100 in PBS for 2 min. (see Lopez-Soler reference); different customer have used this antibody at different dilutions for ICC/IF (see images below). We recommend that optimal working dilutions are determined by each customer. |
画像
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ab24609 staining Nuclear Pore Complex Proteins in Human chronic myelogenous leukemia cells from bone marrow cells. Cells were fixed with 4% paraformaldehyde in DPBS buffer for 20 mins and permeabilised with 0.1% Triton X-100 for 20 min at room temperature. Cells were blocked with 2% normal goat serum in DPBS with 1% BSA and washed with 0.1% Tween 20.
This image was generated using the ascites version of the product.
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ab24609 (1/200) staininig Nuclear Pore Complex Proteins in human RPE-1 cells (green). Cells were fixed in paraformaldehyde, permeabilised with Triton X100 and counterstained with DAPI in order to highlight the nucleus (blue). Please refer to abreview for further experimental details.
This image was generated using the ascites version of the product.
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ab24609 (1/500) staining Nuclear Pore Complex Proteins in human Hela Cells (green). Cells were fixed with Paraformaldehyde/Triton X-100 (10 min in PTEMF buffer (20mM PIPES, 1mM MgCl2, 10mM EGTA, 4% PFA) /0.2% Triton-X100 at room T°C) or Methanol (6 min in Methanol -20 °C , followed by 3 washes in 1x PBS) and counterstained with Dapi in order to highligh the nucleus (blue).
This image was generated using the ascites version of the product.
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ab24609 (1/500) staining Nuclear Pore Complex Proteins in murine NIH/3T3 Cells (green). Cells were fixed with Paraformaldehyde/Triton X-100 (10 min in PTEMF buffer (20mM PIPES, 1mM MgCl2, 10mM EGTA, 4% PFA) /0.2% Triton-X100 at room T°C) or Methanol (6 min in Methanol -20 °C , followed by 3 washes in 1x PBS) and counterstained with Dapi in order to highligh the nucleus (blue).
This image was generated using the ascites version of the product.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (161)
ab24609 は 161 報の論文で使用されています。
- Piët ACA et al. Proximity Ligation Mapping of Microcephaly Associated SMPD4 Shows Association with Components of the Nuclear Pore Membrane. Cells 11:N/A (2022). PubMed: 35203325
- Liu S et al. Mad2 promotes Cyclin B2 recruitment to the kinetochore for guiding accurate mitotic checkpoint. EMBO Rep 23:e54171 (2022). PubMed: 35384228
- Rogg M et al. NUP133 Controls Nuclear Pore Assembly, Transcriptome Composition, and Cytoskeleton Regulation in Podocytes. Cells 11:N/A (2022). PubMed: 35455939
- Gleixner AM et al. NUP62 localizes to ALS/FTLD pathological assemblies and contributes to TDP-43 insolubility. Nat Commun 13:3380 (2022). PubMed: 35697676
- Labasse C et al. Severe ACTA1-related nemaline myopathy: intranuclear rods, cytoplasmic bodies, and enlarged perinuclear space as characteristic pathological features on muscle biopsies. Acta Neuropathol Commun 10:101 (2022). PubMed: 35810298