製品の概要

  • 製品名Nuclear Extraction Kit
  • テスト
    1 x 100 test
  • サンプルの種類
    Tissue, Adherent cells, Suspension cells
  • 全工程の試験時間
    1h 00m
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: all Mammals
  • 製品の概要

    Nuclear Extraction Kit (ab113474) provides a simple and selective method along with all necessary reagents for extracting nuclear proteins in just 1 hour for a variety of applications such as western blotting, protein-DNA binding assays, nuclear enzyme assays or any other procedures requiring optimized nuclear proteins. The protocol is fast and easy-to-use, and also isolates very abundant yields of nuclear extract from mammalian cells or tissue samples.

    Not sure if this is the right product for you? Check out our EpiSeeker Sample Preparation Guide for help.

    Compared to other kits that use conventional nuclear extraction methods, the buffers included in ab113474 contain much lower amounts of salts (80% less than conventional kits) and no SDS, which would allow the enzyme activity to be better kept in the nuclear extracts.

  • 特記事項

     

     

  • アプリケーション適用あり: Functional Studiesmore details

製品の特性

    関連製品

    アプリケーション

    Our Abpromise guarantee covers the use of ab113474 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    Functional Studies Use at an assay dependent concentration.

    Nuclear Extraction Kit 画像

    • Nuclear extracts were prepared from MCF-7 cells and the activity of HDACs were measured using different amounts of the extract. The result shown in the figure demonstrates the ab113474's high specificity.

    プロトコール

    Nuclear Extraction Kit (ab113474) 使用論文

    This product has been referenced in:
    • Wang J  et al. Scrapie infection in experimental rodents and SMB-S15 cells decreased the brain endogenous levels and activities of Sirt1. J Mol Neurosci 55:1022-30 (2015). Read more (PubMed: 25391763) »
    • Galoian K  et al. Restoration of desmosomal junction protein expression and inhibition of H3K9-specific histone demethylase activity by cytostatic proline-rich polypeptide-1 leads to suppression of tumorigenic potential in human chondrosarcoma cells. Mol Clin Oncol 3:171-178 (2015). Functional Studies ; Human . Read more (PubMed: 25469290) »

    See all 14 Publications for this product

    Product Wall

    Yes, this kit can be definitely used for frozen tissues. The lab confirmed it.

    Thank you for your inquiry.

    We have two Nuclear Extraction Kits in our catalog at the moment:

    ab113474

    http://www.abcam.com/index.html?datasheet=113474 (or use the following: http://www.abcam.com/index.html?datasheet=11347...

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    This kit will work better with 2 million cells however you can still use this with half a million cells, when using with 500,000 cells please keep the volume of buffers and reagents proportional to 1 million cells.

    We recommend using Bradford ...

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    I contacted the laboratories. They confirmed that PIC is not required for the tissue homogenization preparation protocol as the active proteases in tissue are much less than that in cultured cells.



    I can confirm that if the cytosol is not required for much consideration, DTT can be excluded in pre-lysis buffer too.



    Yes you can use ab113474, EpiSeeker Nuclear Extraction Kit, to purify soluble nuclear proteins from tissue culture cells or tissues and then detect HIF1 alpha in that extract using ab133104, HIF-1 alpha Transcription Factor Assay Kit.

    A rotor-stator homogenizer even at low speed may disrupt the nuclear membrane. We recommend using a Dounce homogenizer for isolating intact nuclei.

    Nuclear Protein extraction and Histone protein extraction from cell samples frozen at -80oC could still work using ab113474 and ab113476. However, the enzyme activity of nuclear extracts from frozen tissues may be much lower than that from fresh tissues.

    Vielen Dank für Ihren Anruf.

    Ich habe Nachricht aus dem Labor erhalten und freue mich Ihnen bestätigen zu können, dass dieses Kit für Co-IP und natives Protein geeignet ist.

    DTT kann weggelassen werden um sicher ...

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    The cytoplasmic fraction is available for collection after addition of ENE1 followed by centrifugation at step 6 of the protocol for cells or step 3 of the protocol for tissue in the Cell Pellet Preparation section. The cytoplasmic fraction does co...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"