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Nuclear Extraction Kit (ab113474) provides a simple and selective method along with all necessary reagents for extracting nuclear proteins in just 1 hour for a variety of applications such as western blotting, protein-DNA binding assays, nuclear enzyme assays or any other procedures requiring optimized nuclear proteins. The protocol is fast and easy-to-use, and also isolates very abundant yields of nuclear extract from mammalian cells or tissue samples.
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Compared to other kits that use conventional nuclear extraction methods, the buffers included in ab113474 contain much lower amounts of salts (80% less than conventional kits) and no SDS, which would allow the enzyme activity to be better kept in the nuclear extracts.
|1000X Protease Inhibitor Cocktail||1 x 110µl|
|10X Pre-Extraction Buffer||1 x 10ml|
|DTT Solution (1000X)||1 x 110µl|
|ENE2 (Extraction Buffer)||1 x 10ml|
Our Abpromise guarantee covers the use of ab113474 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|