Anti-NTH1 抗体 [2660C1a] (ab70726)
Key features and details
- Mouse monoclonal [2660C1a] to NTH1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
製品の概要
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製品名
Anti-NTH1 antibody [2660C1a]
NTH1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [2660C1a] to NTH1 -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant N-terminal fragment of human NTH1
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ポジティブ・コントロール
- Recombinant human NTH1. Whole cell lysates from human HEK293, mouse NIH3T3 and rat F2408 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
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精製度
Protein G purified -
特記事項(精製)
ab70726 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane. -
ポリ/モノ
モノクローナル -
クローン名
2660C1a -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70726の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (3) |
Use a concentration of 0.2 - 2 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 34 kDa).
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特記事項 |
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WB
Use a concentration of 0.2 - 2 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 34 kDa). |
ターゲット情報
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機能
Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site. Required for the repair of both oxidative DNA damage and spontaneous mutagenic lesions. -
組織特異性
Widely expressed with highest levels in heart and lowest levels in lung and liver. -
配列類似性
Belongs to the Nth/MutY family. -
発生段階
Expression levels are regulated during the cell cycle with increased levels during early and mid S-phase. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 4913 Human
- Entrez Gene: 18207 Mouse
- Entrez Gene: 29541 Rat
- Omim: 602656 Human
- SwissProt: P78549 Human
- SwissProt: O35980 Mouse
- Unigene: 66196 Human
- Unigene: 148315 Mouse
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別名
- Bifunctional DNA N glycoslyase/DNA (apurinic or apyrimidinic site) lyase antibody
- DNA glycoslyase/AP lyase antibody
- Endonuclease III like protein 1 antibody
see all
画像
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All lanes : Anti-NTH1 antibody [2660C1a] (ab70726) at 0.2 µg/ml
Lane 1 : Whole cell lysate from human HEK293 cells
Lane 2 : Whole cell lysate from mouse NIH3T3 cells
Lane 3 : Whole cell lysate from rat F2408 cells
Lysates/proteins at 25 µg per lane.
Predicted band size: 34 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted? -
Anti-NTH1 antibody [2660C1a] (ab70726) at 0.2 µg/ml + immunised recombinant human NTH1 protein
Predicted band size: 34 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (2)
ab70726 は 2 報の論文で使用されています。
- Williams SC & Parsons JL NTH1 Is a New Target for Ubiquitylation-Dependent Regulation by TRIM26 Required for the Cellular Response to Oxidative Stress. Mol Cell Biol 38:N/A (2018). PubMed: 29610152
- Limpose KL et al. Overexpression of the base excision repair NTHL1 glycosylase causes genomic instability and early cellular hallmarks of cancer. Nucleic Acids Res 46:4515-4532 (2018). PubMed: 29522130