Anti-NTF97/Importin beta 抗体 (ab115882)

製品の概要

  • 製品名Anti-NTF97/Importin beta antibody
    NTF97/Importin beta 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to NTF97/Importin beta
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Dog
  • 免疫原

    Synthetic peptide (C-

    RRSKTNKAKTLAT

    ) corresponding to amino acids 851-863 from C terminus of human NTF97/Importin beta

  • ポジティブ・コントロール
    • Daudi cell lysate (WB), Human Spleen tissue (IHC-P)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab115882 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.03 - 0.1 µg/ml. Predicted molecular weight: 97 kDa.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能Functions in nuclear protein import, either in association with an adapter protein, like an importin-alpha subunit, which binds to nuclear localization signals (NLS) in cargo substrates, or by acting as autonomous nuclear transport receptor. Acting autonomously, serves itself as NLS receptor. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates autonomously the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In association with IPO7 mediates the nuclear import of H1 histone. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev. Imports PRKCI into the nucleus.
  • 配列類似性Belongs to the importin beta family.
    Contains 8 HEAT repeats.
    Contains 1 importin N-terminal domain.
  • 細胞内局在Cytoplasm. Nucleus envelope.
  • Information by UniProt
  • 参照データベース
  • 別名
    • IMB 1 antibody
    • IMB1 antibody
    • IMB1_HUMAN antibody
    • Impnb antibody
    • Importin 90 antibody
    • Importin beta 1 antibody
    • Importin beta 1 subunit antibody
    • Importin subunit beta-1 antibody
    • Importin-90 antibody
    • IPOB antibody
    • Karyopherin beta 1 antibody
    • Karyopherin beta 1 subunit antibody
    • Karyopherin subunit beta-1 antibody
    • KPNB 1 antibody
    • Kpnb1 antibody
    • MGC2155 antibody
    • MGC2156 antibody
    • MGC2157 antibody
    • NTF 97 antibody
    • NTF97 antibody
    • Nuclear factor P97 antibody
    • Pore targeting complex 97 kDa subunit antibody
    • PTAC97 antibody
    see all

Anti-NTF97/Importin beta antibody 画像

  • Anti-NTF97/Importin beta antibody (ab115882) at 0.03 µg/ml + Daudi cell lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 97 kDa
  • Human Spleen (formalin-fixed, paraffin-embedded) stained with ab115882 at 5 ug/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-NTF97/Importin beta antibody (ab115882) 使用論文

ab115882 has not yet been referenced specifically in any publications.

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