1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. or Tris-EDTA buffer ( PH8.0)
NTAN1 is required for the ubiquitin-dependent turnover of intracellular proteins that initiate with Met-Asn. This enzyme does not act on substrates with internal or C-terminal asparagines and does not act on glutamine residues in any position.