Anti-Nsr1p 抗体 [31C4] (ab4642)
Key features and details
- Mouse monoclonal [31C4] to Nsr1p
- Suitable for: ICC/IF, WB
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG
製品の概要
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製品名
Anti-Nsr1p antibody [31C4] -
製品の詳細
Mouse monoclonal [31C4] to Nsr1p -
由来種
Mouse -
特異性
This antibody was isolated during a screen for mAbs against yeast nucleolar proteins. Specificity was determined by screening a yeast genomic library in lambda gt11. Two individually isolated, different positive clones were found. Sequencing revealed that in both clones beta-galactosidase was fused at the EcoRI site to the amino acid sequence beginning with …EFEH190 in Nsr1p. Thus, it is likely to react with an epitope(s) in the C-terminal 55% of Nsr1p. -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Saccharomyces cerevisiae -
免疫原
Tissue, cells or virus corresponding to Nsr1p. Yeast nuclear preparation
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.065% Sodium azide
Constituent: Tissue culture supernatant -
Concentration information loading...
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精製度
Tissue culture supernatant -
特記事項(精製)
Sterile filtered. -
ポリ/モノ
モノクローナル -
クローン名
31C4 -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab4642の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC/IF | (1) |
1/500 - 1/5000.
(yeast cells). |
WB | (1) |
1/10000. Detects a band of approximately 66 kDa (predicted molecular weight: 45 kDa).
For non-ECL western detection methods, 1/1000 - 1/5000. |
特記事項 |
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ICC/IF
1/500 - 1/5000. (yeast cells). |
WB
1/10000. Detects a band of approximately 66 kDa (predicted molecular weight: 45 kDa). For non-ECL western detection methods, 1/1000 - 1/5000. |
ターゲット情報
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関連性
Nsr1p is localized in the nucleolus and binds to the single-stranded telomeric repeat sequence (TG1-3)n. It has a role in ribosomal RNA maturation and possibly in transport of proteins into the nucleus. -
細胞内局在
Nuclear -
参照データベース
- Entrez Gene: 853064 Saccharomyces cerevisiae
- SwissProt: P27476 Saccharomyces cerevisiae
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別名
- NSR1 antibody
- Nuclear localization sequence binding protein antibody
- p67 antibody
- SHE5 antibody
画像
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All lanes : Anti-Nsr1p antibody [31C4] (ab4642) at 1/10000 dilution
Lane 1 : Saccharomyces cerevisae DDY1810 (WT) whole cell lysate
Lane 2 : Saccharomyces cerevisae DDY1810 (nsr1 knockout) whole cell lysate
Secondary
All lanes : Rabbit anti-mouse IgG conjugated to peroxidase at 1/30000 dilution
Performed under reducing conditions.
Predicted band size: 45 kDa
Bottom panel: loading control performed using Saccharomyces cerevisae anti-HSP60 (1:1000) -
ab4642 (1/5000) detecting Nsr1p in
DDY1810 (red). Cells were fixed in paraformaldehyde, permeabilized with methanol/acetone and counterstained with DAPI in order to highlight the nucleus (blue).
Right hand panel: negative control performed using Saccharomyces cerevisae DDY1810 nsr1p knockout.
For further experimental details please refer to Abreview.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (2)
ab4642 は 2 報の論文で使用されています。
- Azevedo C et al. Development of a yeast model to study the contribution of vacuolar polyphosphate metabolism to lysine polyphosphorylation. J Biol Chem 295:1439-1451 (2020). PubMed: 31844018
- Lista MJ et al. Nucleolin directly mediates Epstein-Barr virus immune evasion through binding to G-quadruplexes of EBNA1 mRNA. Nat Commun 8:16043 (2017). WB . PubMed: 28685753