製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to NSL1
  • アプリケーション
    適用あり: IP, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rabbit, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide, corresponding to a region between C terminal amino acids 230-281 of Human NSL1 (NP_056286.2)

  • ポジティブ・コントロール
    • Whole cell lysate from HeLa and 293T cells.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7 to 8
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab70836 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at 2-5 µg/mg of lysate.
WB 1/1000 - 1/5000. Detects a band of approximately 38 kDa (predicted molecular weight: 32 kDa).

ターゲット情報

  • 機能
    Part of the MIS12 complex which is required for normal chromosome alignment and segregation and kinetochore formation during mitosis.
  • 翻訳後修飾
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在
    Nucleus. Chromosome > centromere > kinetochore. Associated with the kinetochore.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 4833432M17Rik antibody
    • AI451597 antibody
    • C1orf48 antibody
    • DC31 antibody
    • DC8 antibody
    • DKFZp566O1646 antibody
    • Gm105 antibody
    • Gm984 antibody
    • Kinetochore associated protein NSL1 homolog antibody
    • Kinetochore-associated protein NSL1 homolog antibody
    • MIS14 antibody
    • NSL1 antibody
    • NSL1, MIND kinetochore complex component, homolog (S. cerevisiae) antibody
    • NSL1_HUMAN antibody
    see all

画像

  • Detection of Human NSL1 by Western Blot of Immunoprecipitates in Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells.
    Lane 1: ab70835 used for IP at 3 µg/mg lysate.
    Lane 2: immunoprecipitation with an other antibody
    Lane 3: ab70836 used for IP at 3 µg/mg lysate
    Lane 4: Control IgG IP
    For blotting ab70836 was used at 1 µg/ml.
    Detection: Chemiluminescence with an exposure time of 3 minutes.
  • All lanes : Anti-NSL1 antibody (ab70836) at 0.1 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg

    Developed using the ECL technique

    Predicted band size : 32 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 28 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes

参考文献

ab70836 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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