Detection of Human NSL1 by Western Blot of
Immunoprecipitates in Whole cell lysate (1 mg for IP,
20% of IP loaded) from HeLa cells.
Lane 1: ab70835 used for IP at 3 µg/mg lysate.
Lane 2: immunoprecipitation with an other antibody
Lane 3: ab70836 used for IP at 3 µg/mg lysate
Lane 4: Control IgG IP
For blotting ab70836 was used at 1 µg/ml.
Detection: Chemiluminescence with an exposure time of 3 minutes.
Western blot - Anti-NSL1 antibody (ab70836)
All lanes : Anti-NSL1 antibody (ab70836) at 0.1 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg Lane 2 : Whole cell lysate from HeLa cells at 15 µg Lane 3 : Whole cell lysate from HeLa cells at 5 µg Lane 4 : Whole cell lysate from 293T cells at 50 µg