製品の概要

  • 製品名Anti-NSF antibody [NSF-1]
    NSF 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [NSF-1] to NSF
  • アプリケーション適用あり: ICC/IF, WB, Flow Cyt, IP, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Chicken, Cow, Human
  • 免疫原

    Recombinant full length protein (Human).

  • ポジティブ・コントロール
    • Rat PC12

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab16681 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 10 µg/ml.
WB 1/2000. Detects a band of approximately 76 kDa.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 機能Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seem to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin.
  • 配列類似性Belongs to the AAA ATPase family.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • N ethylmaleimide sensitive factor antibody
    • N ethylmaleimide sensitive factor like protein antibody
    • N ethylmaleimide sensitive fusion protein antibody
    • N-ethylmaleimide-sensitive fusion protein antibody
    • NEM sensitive fusion protein antibody
    • NEM-sensitive fusion protein antibody
    • NSF antibody
    • NSF_HUMAN antibody
    • SKD 2 antibody
    • SKD2 antibody
    • Vesicle fusing ATPase antibody
    • Vesicle-fusing ATPase antibody
    • Vesicular fusion protein NSF antibody
    • Vesicular-fusion protein NSF antibody
    see all

Anti-NSF antibody [NSF-1] 画像

  • All lanes : Anti-NSF antibody [NSF-1] (ab16681) at 0.5 µg/ml

    Lane 1 : 3T3
    Lane 2 : PC12
    Lane 3 : MDBK
    Lane 4 : Normal human fibroblasts
  • ICC/IF image of ab16681 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16681, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing SH-SY5Y cells stained with ab16681 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16681, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG (1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-NSF antibody [NSF-1] (ab16681) 使用論文

This product has been referenced in:
  • Madsen L  et al. Human ASPL/TUG interacts with p97 and complements the proteasome mislocalization of a yeast ubx4 mutant, but not the ER-associated degradation defect. BMC Cell Biol 15:31 (2014). Human . Read more (PubMed: 25078495) »
  • Poirrier JE  et al. Proteomic changes in rat hippocampus and adrenals following short-term sleep deprivation. Proteome Sci 6:14 (2008). WB ; Rat . Read more (PubMed: 18498662) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (brain cortex neurons)
Specification brain cortex neurons
Fixative Formaldehyde
Permeabilization Yes - PBS + 0.2% Triton x-100
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C
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投稿 Sep 06 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (brain)
Loading amount 75 µg
Specification brain
Gel Running Conditions Non-reduced Denaturing (12%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: rt°C
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投稿 Aug 29 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Mouse Cell lysate - whole cell (neuronal)
Total protein in input 500 µg
Specification neuronal
Immuno-precipitation step Other - Dynabeads Protein G
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投稿 Apr 18 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Hippocamus Neuronal cells HpL3-4)
Specification Hippocamus Neuronal cells HpL3-4
Fixative Methanol
Permeabilization Yes - PBS + 0.2% Triton x-100
Blocking step FBS as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: rt°C
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投稿 Sep 30 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (neuronal)
Loading amount 50 µg
Specification neuronal
Gel Running Conditions Non-reduced Denaturing (12%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: rt°C
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投稿 Sep 28 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"