製品の概要

  • 製品名Anti-NSE2 antibody [4A2]
    NSE2 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [4A2] to NSE2
  • アプリケーション適用あり: WB, Flow Cyt, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length protein of Human NSE2 produced in E.coli (NP_775956).

  • ポジティブ・コントロール
    • HEK293T cells transfected with pCMV6-ENTRY control and pCMV6-ENTRY NSE2, COS7 cells transiently transfected by pCMV6-ENTRY NSE2, HEK293T cells transfected with overexpress NSE2 plasmid
  • 特記事項Dilute in PBS (pH7.3) before use.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab119074 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500. Predicted molecular weight: 27 kDa.
Flow Cyt 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100.

ターゲット情報

  • 機能E3 SUMO-protein ligase component of the SMC5-SMC6 complex, a complex involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. Acts as a E3 ligase mediating SUMO attachment to various proteins such as SMC6L1 and TRAX, and maybe the cohesin components RAD21 and STAG2. SUMO protein-ligase activity is required for the prevention of DNA damage-induced apoptosis by facilitating DNA repair.
  • パスウェイProtein modification; protein sumoylation.
  • 配列類似性Belongs to the NSE2 family.
    Contains 1 SP-RING-type zinc finger.
  • 翻訳後修飾Sumoylated, possibly via autosumoylation.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • C8orf36 antibody
    • E3 SUMO-protein ligase NSE2 antibody
    • hMMS21 antibody
    • hNSE2 antibody
    • methyl methanesulfonate sensitivity gene 21 antibody
    • MMS21 antibody
    • MMS21 homolog antibody
    • non-SMC element 2 antibody
    • Non-SMC element 2 homolog antibody
    • Non-structural maintenance of chromosomes element 2 homolog antibody
    • NSE2_HUMAN antibody
    • Nsmce2 antibody
    see all

Anti-NSE2 antibody [4A2] 画像

  • All lanes : Anti-NSE2 antibody [4A2] (ab119074) at 1/500 dilution

    Lane 1 : HEK293T cells transfected with pCMV6-ENTRY control
    Lane 2 : HEK293T cells transfected with pCMV6-ENTRY NSE2

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 27 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC202447)
  • Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NSE2 using ab119074 at a dilution of 1/100.
  • HEK293T cells transfected with either NSE2 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by ab119074 at a dilution of 1/100, and then analyzed by flow cytometry.

Anti-NSE2 antibody [4A2] (ab119074) 使用論文

ab119074 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab119074.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"