製品の概要

  • 製品名Anti-NSE antibody [5E2]
    NSE 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [5E2] to NSE
  • アプリケーション適用あり: ELISA, WB, IHC-P, Flow Cyt, Sandwich ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length native protein (purified) (Human).

  • 特記事項Concentration varies from lot to lot and can be provided on request.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab10171 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration. Can be paired for ELISA with Mouse monoclonal [1C1] to NSE (ab8328).
WB Use at an assay dependent concentration. Detects a band of approximately 45 kDa.
IHC-P 1/100 - 1/300. Antigen retrieval is not essential but may optimise staining.
Flow Cyt Use 0.1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
Sandwich ELISA Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [1C1] to NSE (HRP) (ab24472) and Mouse monoclonal [1C1] to NSE (ab8328). Can be used as capture antibody in conjunction with ab8328. Can be paired for sandwich ELISA with ab24472

ターゲット情報

  • 機能Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.
  • 組織特異性The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
  • パスウェイCarbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
  • 配列類似性Belongs to the enolase family.
  • 発生段階During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.
  • 細胞内局在Cytoplasm. Cell membrane. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 2 phospho D glycerate hydrolyase antibody
    • 2-phospho-D-glycerate hydro-lyase antibody
    • Eno 2 antibody
    • ENO2 antibody
    • ENOG antibody
    • ENOG_HUMAN antibody
    • Enolase 2 (gamma, neuronal) antibody
    • Enolase 2 antibody
    • Enolase 2 gamma neuronal antibody
    • Enolase2 antibody
    • Epididymis secretory protein Li 279 antibody
    • Gamma enolase antibody
    • Gamma-enolase antibody
    • HEL S 279 antibody
    • Neural enolase antibody
    • Neuron specific enolase antibody
    • Neuron specific gamma enolase antibody
    • Neuron-specific enolase antibody
    • Neurone specific enolase antibody
    • NSE antibody
    see all

Anti-NSE antibody [5E2] 画像

  • Overlay histogram showing SH-SH5Y cells stained with ab10171 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab10171, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-NSE antibody [5E2] (ab10171) 使用論文

ab10171 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"