Anti-NSE 抗体 [1C1] (HRP) (ab24472)

製品の概要

  • 製品名Anti-NSE antibody [1C1] (HRP)
    NSE 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [1C1] to NSE (HRP)
  • 標識HRP
  • 特異性Reacts with 45 kD band corresponding to NSE in immunoblotting of human brain extracts
  • アプリケーション適用あり: ELISA, Sandwich ELISA, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Purified Neuron Specific Enolase from human brain.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab24472 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent dilution.
Sandwich ELISA Use at an assay dependent dilution. Can be paired for Sandwich ELISA with Mouse monoclonal [5A4] to NSE (ab10169) and Mouse monoclonal [5E2] to NSE (ab10171) and Mouse monoclonal [5G10] to NSE (ab8324). Can be used as detection antibody in conjunction with recommended pair(s).
WB 1/1000.

ターゲット情報

  • 機能Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.
  • 組織特異性The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
  • パスウェイCarbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
  • 配列類似性Belongs to the enolase family.
  • 発生段階During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.
  • 細胞内局在Cytoplasm. Cell membrane. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 2 phospho D glycerate hydrolyase antibody
    • 2-phospho-D-glycerate hydro-lyase antibody
    • Eno 2 antibody
    • ENO2 antibody
    • ENOG antibody
    • ENOG_HUMAN antibody
    • Enolase 2 (gamma, neuronal) antibody
    • Enolase 2 antibody
    • Enolase 2 gamma neuronal antibody
    • Enolase2 antibody
    • Epididymis secretory protein Li 279 antibody
    • Gamma enolase antibody
    • Gamma-enolase antibody
    • HEL S 279 antibody
    • Neural enolase antibody
    • Neuron specific enolase antibody
    • Neuron specific gamma enolase antibody
    • Neuron-specific enolase antibody
    • Neurone specific enolase antibody
    • NSE antibody
    see all

Anti-NSE antibody [1C1] (HRP) 画像

  • All lanes : Anti-NSE antibody [1C1] (HRP) (ab24472) at 1/1000 dilution

    Lane 2 : Recombinant Human NSE protein (ab78797) at 5 µg
    Lane 3 : Recombinant Human NSE protein (ab78797) at 0.005 µg
    Lane 4 : Whole tissue lysate prepared from human brain at 15 µg

    Developed using the ECL technique

    Observed band size : 45 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    Image courtesy of Dr Rachel Barker by Abreview.

    See Abreview

Anti-NSE antibody [1C1] (HRP) (ab24472) 使用論文

ab24472 has not yet been referenced specifically in any publications.

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I have investigated our records for ab24472 with my colleagues and the originator. I can confirm that regrettably there is an error on the datasheet, and this antibody does not contain sodium azide.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (brain)
Loading amount 15 µg
Specification brain
Gel Running Conditions Reduced Denaturing (4-20% Tris-HCl)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Dr. Rachel Barker

Verified customer

投稿 Mar 21 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"