製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to NSBP1
  • アプリケーション
    適用あり: IHC-P, WB, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide (Human)

  • ポジティブ・コントロール
    • HepG2 cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab56031 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration. PubMed: 22420896
WB Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/62500.

ターゲット情報

  • 機能
    Preferentially binds to euchromatin and modulates cellular transcription by counteracting linker histone-mediated chromatin compaction.
  • 組織特異性
    Ubiquitously expressed.
  • 配列類似性
    Belongs to the HMGN family.
  • ドメイン
    Specifically targeted by its C-terminus to nucleosomes in euchromatin.
  • 細胞内局在
    Nucleus. Associates with nucleosomes in euchromatin and is largely excluded from constitutive heterochromatin.
  • Information by UniProt
  • 参照データベース
  • 別名
    • High mobility group nucleosome binding domain 5 antibody
    • High mobility group nucleosome-binding domain-containing protein 5 antibody
    • Hmgn5 antibody
    • HMGN5_HUMAN antibody
    • NBP 45 antibody
    • NSBP 1 antibody
    • NSBP1 antibody
    • Nucleosomal binding protein 1 antibody
    • Nucleosome binding protein 1 antibody
    • Nucleosome-binding protein 1 antibody
    see all

画像

  • Anti-NSBP1 antibody (ab56031) at 1 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 32 kDa
    Observed band size : 32 kDa

参考文献

This product has been referenced in:
  • Main H  et al. Notch signaling maintains neural rosette polarity. PLoS One 8:e62959 (2013). IHC ; Mouse . Read more (PubMed: 23675446) »
  • Ji SQ  et al. Knockdown of the nucleosome binding protein 1 inhibits the growth and invasion of clear cell renal cell carcinoma cells in vitro and in vivo. J Exp Clin Cancer Res 31:22 (2012). IHC-P ; Human . Read more (PubMed: 22420896) »

See all 2 Publications for this product

レビューと Q&A

There are currently no Customer reviews or Questions for ab56031.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録