Anti-NRG3 抗体 [EPR4148] (ab109256)

製品の概要

  • 製品名Anti-NRG3 antibody [EPR4148]
    NRG3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR4148] to NRG3
  • アプリケーション適用あり: WB, Flow Cytmore details
    適用なし: ICC,IHC-P or IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues in the cytoplasmic domain of Human NRG3.

  • ポジティブ・コントロール
    • Fetal brain, SH-SY5Y, C6, mouse brain, and rat brain lysates; Permeabilized C6 cells
  • 特記事項

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab109256 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 56 kDa (predicted molecular weight: 77 kDa).
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 追加情報Is unsuitable for ICC,IHC-P or IP.
  • ターゲット情報

    • 機能Direct ligand for the ERBB4 tyrosine kinase receptor. Binding results in ligand-stimulated tyrosine phosphorylation and activation of the receptor. Does not bind to the EGF receptor, ERBB2 or ERBB3 receptors. May be a survival factor for oligodendrocytes.
    • 組織特異性Highly expressed in most regions of the brain with the exception of corpus callosum. Expressed at lower level in testis. Not detected in heart, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, ovary, small intestine, colon and peripheral blood leukocytes.
    • 配列類似性Belongs to the neuregulin family.
      Contains 1 EGF-like domain.
    • 発生段階Isoform 3 is expressed in fetal brain but not in other fetal tissues.
    • ドメインThe cytoplasmic domain may be involved in the regulation of trafficking and proteolytic processing. Regulation of the proteolytic processing involves initial intracellular domain dimerization.
      ERBB receptor binding is elicited entirely by the EGF-like domain.
    • 翻訳後修飾Proteolytic cleavage close to the plasma membrane on the external face leads to the release of the soluble growth factor form.
      Extensive glycosylation precedes the proteolytic cleavage (By similarity). Isoform 3 is glycosylated.
    • 細胞内局在Secreted; Cell membrane. Does not seem to be active and Cell membrane. Isoform 3 is also proteolytically released as a soluble form.
    • Information by UniProt
    • 参照データベース
    • 別名
      • HRG3 antibody
      • Neuregulin 3 antibody
      • Neuregulin 3 like polypeptide antibody
      • Neuregulin-3 antibody
      • Neuregulin3 antibody
      • NRG-3 antibody
      • Nrg3 antibody
      • NRG3_HUMAN antibody
      • Pro neuregulin 3 antibody
      • pro NRG3 antibody
      • Pro-NRG3 antibody
      see all

    Anti-NRG3 antibody [EPR4148] 画像

    • All lanes : Anti-NRG3 antibody [EPR4148] (ab109256) at 1/1000 dilution

      Lane 1 : Fetal brain lysate
      Lane 2 : SH-SY5Y lysate
      Lane 3 : C6 lysate
      Lane 4 : Mouse brain lysate
      Lane 5 : Rat brain lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 77 kDa
      Observed band size : 56 kDa (why is the actual band size different from the predicted?)
    • Flow cytometric analysis of permeabilized C6 cells using ab109256 (red) or a rabbit IgG (negative) (green).

    Anti-NRG3 antibody [EPR4148] (ab109256) 使用論文

    ab109256 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (brain)
    Specification brain
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative Formaldehyde
    Username

    Tanveer Ahmed

    Verified customer

    投稿 Jul 15 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"