製品の概要

  • 製品名Anti-NRG3 antibody
    NRG3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to NRG3
  • アプリケーション適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    A synthetic peptide corresponding to a region within the internal sequence 539-588 (TSINMQLPSRETNPYFNSLEQKDLVGYSSTRASSVPIIPSVGLEETCLQ M) of Human NRG3 (NP_001010848).

  • ポジティブ・コントロール
    • Jurkat Cell Lysate

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)Purified by peptide affinity chromatography method.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab83704 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 4 - 8 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 78 kDa.Can be blocked with NRG3 peptide (ab185897). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能Direct ligand for the ERBB4 tyrosine kinase receptor. Binding results in ligand-stimulated tyrosine phosphorylation and activation of the receptor. Does not bind to the EGF receptor, ERBB2 or ERBB3 receptors. May be a survival factor for oligodendrocytes.
  • 組織特異性Highly expressed in most regions of the brain with the exception of corpus callosum. Expressed at lower level in testis. Not detected in heart, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, ovary, small intestine, colon and peripheral blood leukocytes.
  • 配列類似性Belongs to the neuregulin family.
    Contains 1 EGF-like domain.
  • 発生段階Isoform 3 is expressed in fetal brain but not in other fetal tissues.
  • ドメインThe cytoplasmic domain may be involved in the regulation of trafficking and proteolytic processing. Regulation of the proteolytic processing involves initial intracellular domain dimerization.
    ERBB receptor binding is elicited entirely by the EGF-like domain.
  • 翻訳後修飾Proteolytic cleavage close to the plasma membrane on the external face leads to the release of the soluble growth factor form.
    Extensive glycosylation precedes the proteolytic cleavage (By similarity). Isoform 3 is glycosylated.
  • 細胞内局在Secreted; Cell membrane. Does not seem to be active and Cell membrane. Isoform 3 is also proteolytically released as a soluble form.
  • Information by UniProt
  • 参照データベース
  • 別名
    • HRG3 antibody
    • Neuregulin 3 antibody
    • Neuregulin 3 like polypeptide antibody
    • Neuregulin-3 antibody
    • Neuregulin3 antibody
    • NRG-3 antibody
    • Nrg3 antibody
    • NRG3_HUMAN antibody
    • Pro neuregulin 3 antibody
    • pro NRG3 antibody
    • Pro-NRG3 antibody
    see all

Anti-NRG3 antibody 画像

  • Anti-NRG3 antibody (ab83704) at 1 µg/ml (diluted in 5% skim milk / PBS buffer) + Jurkat Cell Lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 78 kDa
    Observed band size : 78 kDa
    Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain, cortex tissue labelling NRG3 with ab83704 at 4-8µg/ml.

Anti-NRG3 antibody (ab83704) 使用論文

ab83704 has not yet been referenced specifically in any publications.

Product Wall

The NRG3 peptide has been added as item ab185897.


Please let me know if you have any questions, for instance regarding how to block the antibody with the peptide. In general, incubating equal amounts by weight of concentrated antibody and...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"