製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Nrf2
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Rat, Guinea pig, Hamster, Dog, Human
    交差が予測される動物種: Cow, Pig, Orangutan
  • 免疫原

    Synthetic peptide:

    SQRRKEYELEKQKKLEKERQE

    conjugated to KLH by a Cysteine residue linker, corresponding to N terminal amino acids 40-60 of Human Nrf2

  • ポジティブ・コントロール
    • Rat liver microsomes, Mouse liver microsomes, Human liver microsomes, Canine liver microsomes and Hela cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab92946 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
  • 追加情報
    WB: 1/1000 (ECL). Detects a band of approximately 80 kDa (predicted molecular weight: 70 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region.
    • 組織特異性
      Widely expressed. Highest expression in adult muscle, kidney, lung, liver and in fetal muscle.
    • 配列類似性
      Belongs to the bZIP family. CNC subfamily.
      Contains 1 bZIP domain.
    • ドメイン
      Acidic activation domain in the N-terminus, and DNA binding domain in the C-terminus.
    • 翻訳後修飾
      Phosphorylation of Ser-40 by PKC in response to oxidative stress dissociates NFE2L2 from its cytoplasmic inhibitor KEAP1, promoting its translocation into the nucleus.
    • 細胞内局在
      Cytoplasm > cytosol. Nucleus. Cytosolic under unstressed conditions, translocates into the nucleus upon induction by electrophilic agents.
    • Information by UniProt
    • 参照データベース
    • 別名
      • erythroid derived 2 antibody
      • HEBP1 antibody
      • like 2 antibody
      • NF E2 related factor 2 antibody
      • NF-E2-related factor 2 antibody
      • NF2L2_HUMAN antibody
      • NFE2 related factor 2 antibody
      • NFE2-related factor 2 antibody
      • Nfe2l2 antibody
      • Nrf 2 antibody
      • NRF2 antibody
      • Nuclear factor (erythroid derived 2) like 2 antibody
      • Nuclear factor antibody
      • nuclear factor erythroid 2 like 2 antibody
      • Nuclear factor erythroid 2 related factor 2 antibody
      • Nuclear factor erythroid 2-related factor 2 antibody
      • Nuclear factor erythroid derived 2 like 2 antibody
      see all

    画像

    • All lanes : Anti-Nrf2 antibody (ab92946) at 1/1000 dilution

      Lane 1 : Molecular weight markers
      Lane 2 : Rat liver microsomes
      Lane 3 : Mouse liver microsomes
      Lane 4 : Human liver microsomes
      Lane 5 : Canine liver microsomes
      Lane 6 : HeLa cell lysate

      Developed using the ECL technique

      Predicted band size : 70 kDa
      Observed band size : 80 kDa (why is the actual band size different from the predicted?)

    プロトコール

    参考文献

    This product has been referenced in:
    • Soares MA  et al. Restoration of Nrf2 Signaling Normalizes the Regenerative Niche. Diabetes 65:633-46 (2016). Read more (PubMed: 26647385) »
    • Meng X  et al. Hydrogen-rich saline attenuates chemotherapy-induced ovarian injury via regulation of oxidative stress. Exp Ther Med 10:2277-2282 (2015). WB . Read more (PubMed: 26668628) »

    See all 2 Publications for this product

    レビューと Q&A

    Thank you for contacting us and for your interest in our products.

    As far as we are aware, ab92946 has never been tested in ChIP. All tested applications are specified on our datasheets, and these are updated as soon as any new information i...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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