製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Nrf2
  • アプリケーション
    適用あり: IHC-P, WB, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Cow, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human Nrf2.

    (Peptide available as ab71889.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following Human Whole Cell Lysates: TE 671, Ramos, A498, Jurkat - Staurosporine Treated (24hr, 500nM), SK N SH

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab71890 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 68 kDa).Can be blocked with Human Nrf2 peptide (ab71889).
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能
    Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region.
  • 組織特異性
    Widely expressed. Highest expression in adult muscle, kidney, lung, liver and in fetal muscle.
  • 配列類似性
    Belongs to the bZIP family. CNC subfamily.
    Contains 1 bZIP domain.
  • ドメイン
    Acidic activation domain in the N-terminus, and DNA binding domain in the C-terminus.
  • 翻訳後修飾
    Phosphorylation of Ser-40 by PKC in response to oxidative stress dissociates NFE2L2 from its cytoplasmic inhibitor KEAP1, promoting its translocation into the nucleus.
  • 細胞内局在
    Cytoplasm > cytosol. Nucleus. Cytosolic under unstressed conditions, translocates into the nucleus upon induction by electrophilic agents.
  • Information by UniProt
  • 参照データベース
  • 別名
    • erythroid derived 2 antibody
    • HEBP1 antibody
    • like 2 antibody
    • NF E2 related factor 2 antibody
    • NF-E2-related factor 2 antibody
    • NF2L2_HUMAN antibody
    • NFE2 related factor 2 antibody
    • NFE2-related factor 2 antibody
    • Nfe2l2 antibody
    • Nrf 2 antibody
    • NRF2 antibody
    • Nuclear factor (erythroid derived 2) like 2 antibody
    • Nuclear factor antibody
    • nuclear factor erythroid 2 like 2 antibody
    • Nuclear factor erythroid 2 related factor 2 antibody
    • Nuclear factor erythroid 2-related factor 2 antibody
    • Nuclear factor erythroid derived 2 like 2 antibody
    see all

画像

  • All lanes : Anti-Nrf2 antibody (ab71890) at 1 µg/ml

    Lane 1 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
    Lane 2 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 3 : A498 (Human Kidney Carcinoma) Whole Cell Lysate
    Lane 4 : Jurkat Whole Cell Lysate - Staurosporine Treated (24hr, 500nM)
    Lane 5 : SK N SH (Human neuroblastoma) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 68 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab71890 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab71890, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) MCF7 cells at 5µg/ml.

参考文献

This product has been referenced in:
  • Wu T  et al. Prevention of murine lupus nephritis by targeting multiple signaling axes and oxidative stress using a synthetic triterpenoid. Arthritis Rheumatol 66:3129-39 (2014). Read more (PubMed: 25047252) »

See 1 Publication for this product

レビューと Q&A

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Lung)
Specification
Lung
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako Target Retrieval Solution
Permeabilization
No
Blocking step
5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
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Mr. Antibody Solutions

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投稿 Apr 08 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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