製品の概要

  • 製品名Anti-Nrf2 antibody
    Nrf2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Nrf2
  • アプリケーション適用あり: IHC-P, WB, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Cow, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human Nrf2.

    (Peptide available as ab71889.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following Human Whole Cell Lysates: TE 671, Ramos, A498, Jurkat - Staurosporine Treated (24hr, 500nM), SK N SH

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab71890 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 68 kDa).Can be blocked with Human Nrf2 peptide (ab71889).
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region.
  • 組織特異性Widely expressed. Highest expression in adult muscle, kidney, lung, liver and in fetal muscle.
  • 配列類似性Belongs to the bZIP family. CNC subfamily.
    Contains 1 bZIP domain.
  • ドメインAcidic activation domain in the N-terminus, and DNA binding domain in the C-terminus.
  • 翻訳後修飾Phosphorylation of Ser-40 by PKC in response to oxidative stress dissociates NFE2L2 from its cytoplasmic inhibitor KEAP1, promoting its translocation into the nucleus.
  • 細胞内局在Cytoplasm > cytosol. Nucleus. Cytosolic under unstressed conditions, translocates into the nucleus upon induction by electrophilic agents.
  • Information by UniProt
  • 参照データベース
  • 別名
    • erythroid derived 2 antibody
    • HEBP1 antibody
    • like 2 antibody
    • NF E2 related factor 2 antibody
    • NF-E2-related factor 2 antibody
    • NF2L2_HUMAN antibody
    • NFE2 related factor 2 antibody
    • NFE2-related factor 2 antibody
    • Nfe2l2 antibody
    • Nrf 2 antibody
    • NRF2 antibody
    • Nuclear factor (erythroid derived 2) like 2 antibody
    • Nuclear factor antibody
    • nuclear factor erythroid 2 like 2 antibody
    • Nuclear factor erythroid 2 related factor 2 antibody
    • Nuclear factor erythroid 2-related factor 2 antibody
    • Nuclear factor erythroid derived 2 like 2 antibody
    see all

Anti-Nrf2 antibody 画像

  • All lanes : Anti-Nrf2 antibody (ab71890) at 1 µg/ml

    Lane 1 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
    Lane 2 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 3 : A498 (Human Kidney Carcinoma) Whole Cell Lysate
    Lane 4 : Jurkat Whole Cell Lysate - Staurosporine Treated (24hr, 500nM)
    Lane 5 : SK N SH (Human neuroblastoma) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 68 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab71890 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab71890, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) MCF7 cells at 5µg/ml.

Anti-Nrf2 antibody (ab71890) 使用論文

ab71890 has not yet been referenced specifically in any publications.

Product Wall

The stock solution of the Optiblot DTT Reducer (10X) ab119199 in 2M. This means that once diluted 10 times this produces a 200 mM solution. Therefore if you usually use 10 mM with your samples I would suggest diluting this 200 times. The higher concent...

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Thank you once again for your enquiries. While my colleague is away, we have received some further information which I would like to forward to you.

In testing using ab62352, the HepG2 were not treated any differently when looking at Nrf2. W...

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Thank you for your reply. I can understand why you would not want to use milk blocking in this case. However, it seems that from the xxxxx datasheet of the antibody xxxx that the results you are seeing is roughly what they would expect. They obtain a b...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Lung)
Specification Lung
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako Target Retrieval Solution
Permeabilization No
Blocking step 5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
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投稿 Apr 08 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"