製品の概要

  • 製品名Anti-Nrf2 antibody
    Nrf2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Nrf2
  • アプリケーション適用あり: IHC-P, ICC/IF, IHC-Fr, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Chicken, Cow
  • 免疫原

    Synthetic peptide:

    TL YLEVFSMLRD EDGKPYSP

    , corresponding to amino acids 569-588 of Human Nrf2

  • ポジティブ・コントロール
    • Placenta

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab31163 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. PubMed: 17142801
WB 1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa). See Abreview.

ターゲット情報

  • 機能Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region.
  • 組織特異性Widely expressed. Highest expression in adult muscle, kidney, lung, liver and in fetal muscle.
  • 配列類似性Belongs to the bZIP family. CNC subfamily.
    Contains 1 bZIP domain.
  • ドメインAcidic activation domain in the N-terminus, and DNA binding domain in the C-terminus.
  • 翻訳後修飾Phosphorylation of Ser-40 by PKC in response to oxidative stress dissociates NFE2L2 from its cytoplasmic inhibitor KEAP1, promoting its translocation into the nucleus.
  • 細胞内局在Cytoplasm > cytosol. Nucleus. Cytosolic under unstressed conditions, translocates into the nucleus upon induction by electrophilic agents.
  • Information by UniProt
  • 参照データベース
  • 別名
    • erythroid derived 2 antibody
    • HEBP1 antibody
    • like 2 antibody
    • NF E2 related factor 2 antibody
    • NF-E2-related factor 2 antibody
    • NF2L2_HUMAN antibody
    • NFE2 related factor 2 antibody
    • NFE2-related factor 2 antibody
    • Nfe2l2 antibody
    • Nrf 2 antibody
    • NRF2 antibody
    • Nuclear factor (erythroid derived 2) like 2 antibody
    • Nuclear factor antibody
    • nuclear factor erythroid 2 like 2 antibody
    • Nuclear factor erythroid 2 related factor 2 antibody
    • Nuclear factor erythroid 2-related factor 2 antibody
    • Nuclear factor erythroid derived 2 like 2 antibody
    see all

Anti-Nrf2 antibody 画像

  • ab31163 staining Nrf2 in Human lymph node tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 1 hour at 20°C; antigen retrieval was by heat mediation in a citric buffer. Samples were incubated with primary antibody (1/100) for 12 hours at 4°C. A Biotin-conjugated Goat anti-rabbit polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • ICC/IF image of ab31163 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab31163, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-Nrf2 antibody (ab31163) at 1 µg/ml

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : A498 (Human Kidney Carcinoma) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 68 kDa
    Observed band size : 68 kDa
    Additional bands at : 28 kDa,37 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute
  • Immunohistochemical analysis of frozen mouse retinal tissue, labelling Nrf2 with ab31163 at a dilution of 1/100 incubated for 2 hours at 21°C. Permeabilization was via additon of 0.5% Triton X-100. Blocking was with 1% BSA for 1 hour at 21°C. The secondary used was ab150086 used at 1/600.

    See Abreview

  • ab31163 staining Nrf2 in Mouse abdominal wall tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% serum for 1 hour at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/100 in milk) for 18 hours at 4°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • ab31163 at 1/100 dilution, staining Nrf2 in human placenta by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections).

Anti-Nrf2 antibody (ab31163) 使用論文

This product has been referenced in:
  • Guo C  et al. Therapeutic time window and underlying therapeutic mechanism of breviscapine injection against cerebral ischemia/reperfusion injury in rats. J Ethnopharmacol 151:660-6 (2014). WB, IHC-Fr ; Rat . Read more (PubMed: 24291152) »
  • Jo C  et al. Nrf2 reduces levels of phosphorylated tau protein by inducing autophagy adaptor protein NDP52. Nat Commun 5:3496 (2014). Read more (PubMed: 24667209) »

See all 19 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (BxPC3 - pancreatic cancer cell line)
Permeabilization Yes - 0.25% Triton X-100 in PBS
Specification BxPC3 - pancreatic cancer cell line
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Fixative Formaldehyde
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投稿 Jan 18 2016

Application Western blot
Sample Mouse Tissue lysate - other (liver cytosol)
Gel Running Conditions Reduced Denaturing (gel 10%)
Loading amount 40 µg
Treatment hyperoxia (95%O2) for 44 hours
Specification liver cytosol
Blocking step Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: +4°C
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Dr. Sandra Sobocanec

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投稿 Oct 06 2015

Application Western blot
Sample Mouse Tissue lysate - nuclear (retina)
Gel Running Conditions Non-reduced Denaturing
Loading amount 20 µg
Specification retina
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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投稿 Oct 01 2015

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (retina)
Permeabilization Yes - 0.5% Triton X-100
Specification retina
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative fresh frozen
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投稿 Aug 17 2015

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - nuclear (Spinal cord lysate)
Specification Spinal cord lysate
Blocking step Licor Blocking buffer as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 100%
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投稿 Jan 14 2015

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (12%)
Sample Mouse Cell lysate - whole cell (brain)
Specification brain
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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投稿 Aug 22 2014



I believe that the Nrf2 that does enter the nucleus is acetylated and phosphorylated, which will make the protein run more slowly through the SDS PAGE gel, making its apparent molecular weight seem larger than based on theoretical amino acid ...

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Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (10% gel)
Sample Human Cell lysate - whole cell (HepG2 cell lysate-ab7900)
Specification HepG2 cell lysate-ab7900
Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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投稿 Mar 07 2014

After review of your protocol, at this time, I would recommend use of 3-5% BSA in TBST blocking solution or to use a casein block. There are two other possibilities which could help as well. The first is that there may be too much protein per well. ...

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Knocking down the gene in a cell line is a good way to determine specificity. I would also recommend adjusting antibody concentration and blocking conditions, prehaps use of BSA instead of milk, to attempt to reduce the non specific binding which you ...

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1-10 of 39 Abreviews or Q&A

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