製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to Nrf2
  • アプリケーション
    適用あり: WBmore details
  • 種交差性

    交差が予測される動物種: Mouse, Rat, Cow, Dog, Human, Pig
  • 免疫原

    Synthetic peptide:

    C-KHSSRLEAHLTRDE

    with a Cysteine residue linker, corresponding to internal sequence amino acids 445-458 of Human Nrf2 (NP_006155.2; NP_001138884.1; NP_001138885.1).

  • ポジティブ・コントロール
    • Human lung lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab121035 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 68 kDa).

ターゲット情報

  • 機能
    Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region.
  • 組織特異性
    Widely expressed. Highest expression in adult muscle, kidney, lung, liver and in fetal muscle.
  • 配列類似性
    Belongs to the bZIP family. CNC subfamily.
    Contains 1 bZIP domain.
  • ドメイン
    Acidic activation domain in the N-terminus, and DNA binding domain in the C-terminus.
  • 翻訳後修飾
    Phosphorylation of Ser-40 by PKC in response to oxidative stress dissociates NFE2L2 from its cytoplasmic inhibitor KEAP1, promoting its translocation into the nucleus.
  • 細胞内局在
    Cytoplasm > cytosol. Nucleus. Cytosolic under unstressed conditions, translocates into the nucleus upon induction by electrophilic agents.
  • Information by UniProt
  • 参照データベース
  • 別名
    • erythroid derived 2 antibody
    • HEBP1 antibody
    • like 2 antibody
    • NF E2 related factor 2 antibody
    • NF-E2-related factor 2 antibody
    • NF2L2_HUMAN antibody
    • NFE2 related factor 2 antibody
    • NFE2-related factor 2 antibody
    • Nfe2l2 antibody
    • Nrf 2 antibody
    • NRF2 antibody
    • Nuclear factor (erythroid derived 2) like 2 antibody
    • Nuclear factor antibody
    • nuclear factor erythroid 2 like 2 antibody
    • Nuclear factor erythroid 2 related factor 2 antibody
    • Nuclear factor erythroid 2-related factor 2 antibody
    • Nuclear factor erythroid derived 2 like 2 antibody
    see all

画像

  • Anti-Nrf2 antibody (ab121035) at 0.3 µg/ml + Human lung lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 68 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)

プロトコール

参考文献

ab121035 has not yet been referenced specifically in any publications.

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