製品の概要

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab55744 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 54 kDa.
IHC-P Use a concentration of 3 µg/ml.
IP Use at an assay dependent concentration. PubMed: 24250222

ターゲット情報

  • 機能Transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. Links the transcriptional modulation of key metabolic genes to cellular growth and development. Implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication.
  • 組織特異性Ubiquitously expressed with strongest expression in skeletal muscle.
  • 配列類似性Belongs to the NRF1/Ewg family.
  • 翻訳後修飾Phosphorylation enhances DNA binding.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • alpha pal antibody
    • alpha palindromic binding protein antibody
    • Alpha palindromic-binding protein antibody
    • Alpha-pal antibody
    • locus control region factor 1 antibody
    • NFE2 related factor 1 antibody
    • NRF-1 antibody
    • Nrf1 antibody
    • NRF1_HUMAN antibody
    • Nuclear respiratory factor 1 antibody
    see all

Anti-NRF1 antibody 画像

  • Anti-NRF1 antibody (ab55744) at 1 µg/ml + human Skeletal muscle

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate

    Predicted band size : 54 kDa


  • Predicted band size : 54 kDa

    This image is courtesy of an anonymous Abreview.

    Western blot analysis of HEK293 whole cell lysate (30μg/lane) labelling NRF1 with ab55744 at 1/1000 in5% milk for 16 hours at 4ºC. A IRDye® 680-conjugated goat anti-rmouse polyclonal (1/4000) was used as the secondary antibody. Exposure was 5 minutes and Licor scan dection was used.

  • NRF1 antibody (ab55744) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human kidney.

Anti-NRF1 antibody (ab55744) 使用論文

This product has been referenced in:

See all 5 Publications for this product

Product Wall

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - whole cell (liver)
Specification liver
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

投稿 Nov 10 2014

Below please find a new discount code and new expiration date regarding testing of ab55744 in zebrafish. Please let me know if you have any questions.

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I am very pleased to hear you would like to accept our offer and test ab557...

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Application Western blot
Sample Zebrafish Cell lysate - whole cell (ZEB2J)
Loading amount 20 µg
Specification ZEB2J
Gel Running Conditions Reduced Denaturing (10)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

投稿 Jul 03 2012

DISCOUNT CODE: ***
Expiration date: August 19th, 2012

I am very pleased to hear you would like to accept our offer and test ab55744 in zebrafish. This code will give you: 1 free primary antibody before the expiration date. To redeem this...

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Thank you for getting back to me and for providing some further details. It is very much appreciated. It may be worth running human heart or skeletal muscle lysate alongside the samples to see if endogenously expressed NRF1 protein runs differe...

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Thank you for contacting Abcam Technical Team and for taking the time to provide some useful details of the experiments. I am very sorry to hear that your customer is having problems with this product. Though you have kindly provided some detail...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (Muscle, vastus lateralis)
Loading amount 20 µg
Specification Muscle, vastus lateralis
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Dr. Andrew Murray

Verified customer

投稿 Mar 17 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"