Anti-NRF1 抗体 (ab34682)
Key features and details
- Rabbit polyclonal to NRF1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-NRF1 antibody
NRF1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to NRF1 -
由来種
Rabbit -
特異性
ab34682 recognises NRF1 (also known as nuclear respiratory factor 1, alpha palindromic binding protein and alpha-pal). -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Rat, Chicken, Dog, Chimpanzee, Zebrafish -
免疫原
Recombinant full length protein, corresponding to amino acids 1-534 of Mouse NRF1
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab34682の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (3) |
1/500 - 1/2000. Detects a band of approximately 67 kDa (predicted molecular weight: 57 kDa). Splice variants exist for this protein that may result in the detection of lower molecular weight bands.
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IHC-P |
Use a concentration of 2 - 5 µg/ml.
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ICC/IF |
Use a concentration of 1 µg/ml.
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特記事項 |
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WB
1/500 - 1/2000. Detects a band of approximately 67 kDa (predicted molecular weight: 57 kDa). Splice variants exist for this protein that may result in the detection of lower molecular weight bands. |
IHC-P
Use a concentration of 2 - 5 µg/ml. |
ICC/IF
Use a concentration of 1 µg/ml. |
ターゲット情報
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機能
Transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. Links the transcriptional modulation of key metabolic genes to cellular growth and development. Implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. -
組織特異性
Ubiquitously expressed with strongest expression in skeletal muscle. -
配列類似性
Belongs to the NRF1/Ewg family. -
翻訳後修飾
Phosphorylation enhances DNA binding. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 416677 Chicken
- Entrez Gene: 4899 Human
- Entrez Gene: 312195 Rat
- Entrez Gene: 64604 Zebrafish
- Omim: 600879 Human
- SwissProt: Q16656 Human
- SwissProt: Q62792 Rat
- SwissProt: Q90X44 Zebrafish
see all -
別名
- alpha pal antibody
- alpha palindromic binding protein antibody
- Alpha palindromic-binding protein antibody
see all
画像
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All lanes : Anti-NRF1 antibody (ab34682) at 1/1000 dilution
Lane 1 : Mouse liver whole tissue lysate
Lane 2 : HeLa whole cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG polyclonal at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 57 kDa
Observed band size: 55,67 kDa why is the actual band size different from the predicted?
Exposure time: 50 seconds
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All lanes : Anti-NRF1 antibody (ab34682) at 1/500 dilution
Lane 1 : HeLa Nuclear extract
Lane 2 : HeLa Whole Cell lysate
Secondary
All lanes : IRDyeTM700 labelled goat-a-rabbit IgG at 1/2500 dilution
Predicted band size: 57 kDa
Observed band size: 67 kDa why is the actual band size different from the predicted? -
Purified anti-NRF1 antibody (ab34682) was used at 5µg/ml to detect NRF1 in human testis. Nuclear staining in lymphocytes can be observed.
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ICC/IF image of ab34682 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab34682, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (41)
ab34682 は 41 報の論文で使用されています。
- Hwang JH et al. TAZ links exercise to mitochondrial biogenesis via mitochondrial transcription factor A. Nat Commun 13:653 (2022). PubMed: 35115527
- Li L et al. Mitochondrial dynamics and biogenesis indicators may serve as potential biomarkers for diagnosis of myasthenia gravis. Exp Ther Med 23:307 (2022). PubMed: 35340870
- Xiao Z et al. MicroRNA-205-5p plays a suppressive role in the high-fat diet-induced atrial fibrosis through regulation of the EHMT2/IGFBP3 axis. Genes Nutr 17:11 (2022). PubMed: 35858845
- Wang X et al. Testosterone attenuates pulmonary epithelial inflammation in male rats of COPD model through preventing NRF1-derived NF-?B signaling. J Mol Cell Biol 13:128-140 (2021). PubMed: 33475136
- Rajasekaran N et al. Nuclear Respiratory Factor-1, a Novel SMAD4 Binding Protein, Represses TGF-ß/SMAD4 Signaling by Functioning as a Transcriptional Cofactor. Int J Mol Sci 22:N/A (2021). PubMed: 34070531