Anti-NrCAM 抗体 - Neuronal Marker (ab24344)

製品の概要

  • 製品名Anti-NrCAM antibody - Neuronal Marker
    NrCAM 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to NrCAM - Neuronal Marker
  • 特異性ab24344 immunoblots a single major protein of NrCAM (see western blot image on datasheet) and recognizes proteins of comparable size from mouse, rat and human.
  • アプリケーション適用あり: IHC-Fr, IHC-P, ICC/IF, WB, IP, IHC-FoFrmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    mouse NrCAM protein as fusion with human Fc region of Ig. This antibody recognizes an extracellular region of the protein. It cross-reacts with human Fc region

  • 特記事項This antibody recognizes an extracellular region of the protein. It cross-reacts with human Fc region.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab24344 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration.
IHC-P 1/200. Do not perform antigen retrieval.
ICC/IF 1/500.
WB 1/500. Detects a band of approximately 145 kDa (predicted molecular weight: 145 kDa).
IP Use at an assay dependent concentration.
IHC-FoFr 1/200. PubMed: 113319561/200. Tissue was immersion fixed (not perfusion fixed) overnight in 4% paraformaldehyde/PBS pH 7.4 and then cryoprotected by incubation in 30% sucrose overnight. Sections were blocked for 1 hour with 1% goat serum/5% BSA in PBS with 0.02% sodium azide and 0.02% Triton X-100 (see PubMed: 11331956).

ターゲット情報

  • 機能Cell adhesion, ankyrin-binding protein involved in neuron-neuron adhesion. May play a role in the molecular assembly of the nodes of Ranvier.
  • 組織特異性Detected in all the examined tissues. In the brain it was detected in the amygdala, caudate nucleus, corpus callosum, hippocampus, hypothalamus, substantia nigra, subthalamic nucleus and thalamus.
  • 配列類似性Belongs to the immunoglobulin superfamily. L1/neurofascin/NgCAM family.
    Contains 5 fibronectin type-III domains.
    Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
  • 細胞内局在Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Bravo antibody
    • hBravo antibody
    • Neuronal cell adhesion molecule antibody
    • Neuronal surface protein Bravo antibody
    • Ng CAM related antibody
    • Ng-CAM-related antibody
    • NgCAM related cell adhesion molecule antibody
    • NgCAM-related cell adhesion molecule antibody
    • Nr CAM antibody
    • Nr-CAM antibody
    • Nrcam antibody
    • NRCAM_HUMAN antibody
    see all

Anti-NrCAM antibody - Neuronal Marker 画像

  • ab24344 detects NrCAM by immunofluorescence on embryonic day 11.5 mouse spinal cord. Floor plate commissurral axons crossing to the contralateral side of the spinal cord are shown here labeled for NrCAM by ab24344 (1/300). Mouse spinal cord was 4% PFA-fixed and processed on slide for IF. Scale bar=50umab24344 detects NrCAM by immunofluorescence on embryonic day 11.5 mouse spinal cord. Floor plate commissurral axons crossing to the contralateral side of the spinal cord are shown here labeled for NrCAM by ab24344 (1/300). Mouse spinal cord was 4% PFA-fixed and processed on slide for IF. Scale bar=50um
  • Anti-NrCAM antibody - Neuronal Marker (ab24344) at 1/500 dilution + 20 µg of postnatal day 8 mouse brain extract in SDS

    Predicted band size : 145 kDa
    Observed band size : 145 kDa

    This image is courtesy of Martin Grumet, Rutgers University, United States

    ab24344 also can be used for immunoprecipatation and blocks function

Anti-NrCAM antibody - Neuronal Marker (ab24344) 使用論文

This product has been referenced in:
  • Brügger V  et al. HDAC1/2-Dependent P0 Expression Maintains Paranodal and Nodal Integrity Independently of Myelin Stability through Interactions with Neurofascins. PLoS Biol 13:e1002258 (2015). Read more (PubMed: 26406915) »
  • Amor V  et al. Long-term maintenance of Na+ channels at nodes of Ranvier depends on glial contact mediated by gliomedin and NrCAM. J Neurosci 34:5089-98 (2014). Mouse . Read more (PubMed: 24719088) »

See all 16 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Mouse Cell (Fluorescent assisted cell sorting)
Specification Fluorescent assisted cell sorting
Preparation Cell harvesting/tissue preparation method: Cortex digested with Accutase, debris filtered via percoll gradient, cells strained through a 40uM filter and then stained with 1Ab
Sample buffer: RNAse free PBS
Fixation No fixation
Permeabilization No
Gating Strategy neuronal cells were gated
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投稿 Mar 20 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Retina)
Specification Retina
Fixative Paraformaldehyde
Blocking step Other as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10
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投稿 Aug 01 2007

Thank you for your enquiry. For further information about the protein sequence, please refer to the publication below. http://www.jcb.org/cgi/reprint/113/6/1399 Please bear in mind that this product has not been tested in flow cytometry and ...

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Yes, Karen and I spoke and decided on the advice Karen gave to you. I have still have the same comments. What results did you obtain from the no primary control you ran? If you are still seeing the high background with the secondary only, have you tr...

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Thank you for your reply and attaching the images. You have a very poor result but at this point I have no way of determining if the antibody is causing the problem or not. It would be highly unusual that an antibody would yield a pattern of staining...

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I'm sorry to hear you are having a problem with ab24344. I would like to suggest the following modifications to your protocol: Please use a fresh blot and try: blocking in 5% milk for 30 min, incubating in primary overnight in 5% milk at 4 degrees...

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