This antibody is predicted to recognize isoforms 1-5 of NrCAM. The predicted molecular weights of isoforms 1, 2, 3, 4 and 5 are 144, 137, 131, 131 and 144 kDa respectively. We propose that the band at 130kDa corresponds to either isoform 3 or isoform 4 of NrCAM. Also, the first 24 amino acids of the NrCAM sequence act as a signal sequence, and when cleaved the protein has an expected molecular weight of approximately 128 kDa.
IHC image of 87427 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab87427, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.