製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to NRBF2
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WB, IPmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residue 237 and 287 of Human NRBF2 (NP_110386.2).

  • ポジティブ・コントロール
    • Whole cell lysate from HeLa, 293T and mouse NIH3T3 cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab85733 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 32 kDa.
IP Use at 2-5 µg/mg of lysate.

ターゲット情報

画像

  • All lanes : Anti-NRBF2 antibody (ab85733) at 0.4 µg/ml

    Lane 1 : Whole cell
    lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell
    lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell
    lysate from HeLa cells at 5 µg
    Lane 4 : Whole cell
    lysate from 293T cells at 50 µg
    Lane 5 : Whole cell
    lysate from NIH3T3 cells at 50 µg

    Developed using the ECL technique.

    Predicted band size: 32 kDa
    Observed band size: 40 kDa (why is the actual band size different from the predicted?)


    Exposure time: 30 seconds
  • Detection of Human NRBF2 in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab85733 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.

参考文献

ab85733 has not yet been referenced specifically in any publications.

レビューと Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (brain)
Permeabilization
Yes - triton X-100
Specification
brain
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 0.2% · Temperature: RT°C
Fixative
Formaldehyde
Username

Xiaosen Ouyang

Verified customer

投稿 Dec 08 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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