製品の概要

  • 製品名Anti-NRBF2 antibody
    NRBF2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to NRBF2
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residue 237 and 287 of Human NRBF2 (NP_110386.2).

  • ポジティブ・コントロール
    • Whole cell lysate from HeLa, 293T and mouse NIH3T3 cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab85733 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 32 kDa.
IP Use at 2-5 µg/mg of lysate.

ターゲット情報

  • 機能May modulate transcriptional activation by target nuclear receptors. Can act as transcriptional activator (in vitro).
  • 組織特異性Detected in keratinocytes, liver and placenta.
  • 細胞内局在Nucleus. Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Comodulator of PPAR and RXR 1 antibody
    • Comodulator of PPAR and RXR 2 antibody
    • Comodulator of PPAR and RXR antibody
    • COPR antibody
    • COPR1 antibody
    • COPR2 antibody
    • DKFZp564C1664 antibody
    • FLJ30395 antibody
    • NRBF 2 antibody
    • NRBF-2 antibody
    • NRBF2 antibody
    • NRBF2_HUMAN antibody
    • Nuclear receptor binding factor 2 antibody
    • Nuclear receptor-binding factor 2 antibody
    see all

Anti-NRBF2 antibody 画像

  • All lanes : Anti-NRBF2 antibody (ab85733) at 0.4 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg
    Lane 5 : Whole cell lysate from NIH3T3 cells at 50 µg

    Developed using the ECL technique

    Predicted band size : 32 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds
  • Detection of Human NRBF2 in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab85733 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.

Anti-NRBF2 antibody (ab85733) 使用論文

ab85733 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"