Rabbit polyclonal to NRAP
internal sequence amino acids 1309-1393 (RHDFVKERGK LIGPQSVRDD PRIQHCRRMG QLQSELQYRR GATSSQAQFH LPMDMVHLVH AKNAQALASD HDYRTQYHKF TALPE) of the Human NRAP protein (UniProt ID: Q86VF7).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
May be involved in anchoring the terminal actin filaments in the myofibril to the membrane and in transmitting tension from the myofibrils to the extracellular matrix.
Expressed in cardiac and skeletal muscle.
Contains 1 LIM zinc-binding domain.
Contains 44 nebulin repeats.
Localized at the myotendinous junction in skeletal muscle and at the intercalated disk in cardiac muscle.
Information by UniProt
Nebulin-related-anchoring protein antibody
Immunocytochemistry/ Immunofluorescence - Anti-NRAP antibody (ab122427)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli, centrosome and cell junctions. Recommended concentration of ab122427 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NRAP antibody (ab122427)
ab122427, at a 1/200 dilution, staining NRAP in paraffin embedded Human liver tissue by Immunohistochemistry.
Western blot - Anti-NRAP antibody (ab122427) This image is courtesy of an anonymous abreview.
Anti-NRAP antibody (ab122427) at 1/1000 dilution + Sheep cardiac tissue at 10 µg
Donkey Anti-Rabbit IgG (H+L) at 1/20000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Observed band size :
190 kDa (
why is the actual band size different from the predicted?
Exposure time :
This image is courtesy of an anonymous abreview.
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