製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to NRAMP1
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Rat, Human
    交差が予測される動物種: Mouse, Sheep, Rabbit, Goat, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide: FARVLLTRSC AILPTVLVAV FRDLRDLSGL NDLLNVLQSL LLPFAVLPIL , corresponding to amino acids 398-447 of Human NRAMP1

  • ポジティブ・コントロール
    • HepG2 membrane lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab59696 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1:1562500.

ターゲット情報

  • 機能
    Divalent transition metal (iron and manganese) transporter involved in iron metabolism and host resistance to certain pathogens. Macrophage-specific membrane transport function. Controls natural resistance to infection with intracellular parasites. Pathogen resistance involves sequestration of Fe(2+) and Mn(2+), cofactors of both prokaryotic and eukaryotic catalases and superoxide dismutases, not only to protect the macrophage against its own generation of reactive oxygen species, but to deny the cations to the pathogen for synthesis of its protective enzymes.
  • 組織特異性
    Macrophages; peripheral blood leukocytes, lung, spleen and liver.
  • 配列類似性
    Belongs to the NRAMP family.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • LSH antibody
    • Natural resistance associated macrophage protein 1 antibody
    • Natural resistance-associated macrophage protein 1 antibody
    • NRAM1_HUMAN antibody
    • NRAMP 1 antibody
    • NRAMP antibody
    • PBC antibody
    • SLC11A1 antibody
    • Solute carrier family 11 (proton coupled divalent metal ion transporters) member 1 antibody
    • solute carrier family 11 (sodium/phosphate symporters) member 1 antibody
    • Solute carrier family 11 member 1 antibody
    see all

画像

  • Anti-NRAMP1 antibody (ab59696) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 60 kDa
    Observed band size : 60 kDa

プロトコール

参考文献

ab59696 has not yet been referenced specifically in any publications.

レビューと Q&A

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (12%)
Sample
Rat Tissue lysate - whole (Liver)
Specification
Liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

投稿 Jun 17 2013

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Tissue lysate - whole (Liver)
Specification
Liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

投稿 Jun 17 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (Liver)
Loading amount
50 µg
Specification
Liver
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

投稿 Sep 06 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HepG2)
Loading amount
50 µg
Specification
HepG2
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Aug 22 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HepG2)
Loading amount
50 µg
Specification
HepG2
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

投稿 Dec 07 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (Liver)
Loading amount
50 µg
Specification
Liver
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

投稿 Sep 09 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HepG2)
Loading amount
20 µg
Specification
HepG2
Gel Running Conditions
Reduced Denaturing (12% Bis-Tris)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. Shakil Ahmad

Verified customer

投稿 Aug 08 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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