製品の概要

  • 製品名Anti-NRAMP1 antibody
    NRAMP1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to NRAMP1
  • アプリケーション適用あり: WB, ICC/IF, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment: QAFYQKTNQA AFNICANSSL HDYAKIFPMN NATVAVDIYQ GGV, corresponding to amino acids 308-351 of Human NRAMP1

  • ポジティブ・コントロール
    • This antibody gave a positive result when used in the following methanol fixed cell lines: A549

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab58138 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml.

This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

ICC/IF Use a concentration of 5 µg/ml.
Flow Cyt Use 0.1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Divalent transition metal (iron and manganese) transporter involved in iron metabolism and host resistance to certain pathogens. Macrophage-specific membrane transport function. Controls natural resistance to infection with intracellular parasites. Pathogen resistance involves sequestration of Fe(2+) and Mn(2+), cofactors of both prokaryotic and eukaryotic catalases and superoxide dismutases, not only to protect the macrophage against its own generation of reactive oxygen species, but to deny the cations to the pathogen for synthesis of its protective enzymes.
  • 組織特異性Macrophages; peripheral blood leukocytes, lung, spleen and liver.
  • 配列類似性Belongs to the NRAMP family.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • LSH antibody
    • Natural resistance associated macrophage protein 1 antibody
    • Natural resistance-associated macrophage protein 1 antibody
    • NRAM1_HUMAN antibody
    • NRAMP 1 antibody
    • NRAMP antibody
    • PBC antibody
    • SLC11A1 antibody
    • Solute carrier family 11 (proton coupled divalent metal ion transporters) member 1 antibody
    • solute carrier family 11 (sodium/phosphate symporters) member 1 antibody
    • Solute carrier family 11 member 1 antibody
    see all

Anti-NRAMP1 antibody 画像

  • Overlay histogram showing HepG2 cells stained with ab58138 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab58138, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was an anti-mouse Alexa Fluor® 488 (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

  • ICC/IF image of ab58138 stained A549 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab58138 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Western blot against tagged recombinant protein immunogen using ab58138 NRAMP1 antibody at 1ug/ml. Predicted band size of immunogen is 31 kDa

Anti-NRAMP1 antibody (ab58138) 使用論文

ab58138 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"