製品の概要

  • 製品名
    Anti-NR5A2 / LRH1 antibody
    NR5A2 / LRH1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to NR5A2 / LRH1
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rat, Sheep, Guinea pig, Cow, Pig, Chimpanzee
  • 免疫原

    A region within synthetic peptide: MSSNSDTGDL QESLKHGLTP IVSQFKMVNY SYDEDLEELC PVCGDKVSGY, corresponding to N terminal amino acids 1-50 of Human NR5A2/ LRH1

  • ポジティブ・コントロール
    • HepG2 cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab62601 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 61 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/12500.

ターゲット情報

  • 機能
    Binds to the sequence element 5'-AACGACCGACCTTGAG-3' of the enhancer II of hepatitis B virus genes, a critical cis-element of their expression and regulation. May be responsible for the liver-specific activity of enhancer II, probably in combination with other hepatocyte transcription factors. Key regulator of cholesterol 7-alpha-hydroxylase gene (CYP7A) expression in liver. May also contribute to the regulation of pancreas-specific genes and play important roles in embryonic development.
  • 組織特異性
    Abundantly expressed in pancreas, less in liver, very low levels in heart and lung. Expressed in the Hep-G2 cell line. Isoform 1 and isoform 2 seem to be present in fetal and adult liver and Hep-G2 cells.
  • 配列類似性
    Belongs to the nuclear hormone receptor family. NR5 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Alpha-1-fetoprotein transcription factor antibody
    • B1-binding factor antibody
    • B1F antibody
    • B1F2 antibody
    • CPF antibody
    • CYP7A promoter-binding factor antibody
    • FTF antibody
    • FTZ F1 antibody
    • FTZ F1beta antibody
    • FTZ-F1 antibody
    • FTZ-F1beta antibody
    • hB1F 2 antibody
    • hB1F antibody
    • hB1F-2 antibody
    • Hepatocytic transcription factor antibody
    • Liver receptor homolog 1 antibody
    • LRH-1 antibody
    • LRH1 antibody
    • Nr5a2 antibody
    • NR5A2_HUMAN antibody
    • Nuclear receptor subfamily 5 group A member 2 antibody
    see all

画像

  • Lane 1 : Marker
    Lane 2 : Anti-NR5A2 / LRH1 antibody (ab62601) at 2.5 µg/ml

    Lane 1 : As above
    Lane 2 : HepG2 cell lysate at 10 µg

    Secondary
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 61 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)

プロトコール

参考文献

ab62601 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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