製品の概要

  • 製品名
    Anti-NR2F2 antibody [H7147]
    NR2F2 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [H7147] to NR2F2
  • 由来種
    Mouse
  • 特異性
    This antibody does not recognize human COUP TF1 and EAR2.
  • アプリケーション
    適用あり: ELISA, IHC-P, IP, ICC/IF, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Cow
  • 免疫原

    Recombinant fragment:

    PGQGGPAS TPAQTAAGGQ GGPG

    , corresponding to amino acids 43-64 of Human NR2F2

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • ポジティブ･コントロール
    • Human Convoluted tubule and Rat Glomerular

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab41859 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use a concentration of 0.1 µg/ml.
IHC-P Use a concentration of 10 µg/ml.
IP Use at an assay dependent concentration.
ICC/IF 1/1000.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 46 kDa).

ターゲット情報

画像

  • Immunocytochemistry/ Immunofluorescence - Anti-NR2F2 antibody [H7147] (ab41859)
    Immunocytochemistry/ Immunofluorescence - Anti-NR2F2 antibody [H7147] (ab41859)Image is courtesy of an anonymous Abreview.

    Immnocytochemical immunoflurescence analysis of PFA-fixed human pluripotent stem cell derived cardiomyocytes, labelling NR2F2 with ab41859 at a concentration of 1/1000 incubated for 16 hours at 4°C. Permeablisation is using saponin. Blocking was done with 5% serum for 15 minutes at room temperature. Secondary used was a goat anti-mouse Alexa Fluor® 568 at 1/2000. The left panel shows the experimental conditions described formerly. The right panel is additionally counterstained with DAPI to show all cell nuclei within the area.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR2F2 antibody [H7147] (ab41859)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR2F2 antibody [H7147] (ab41859)
    ab41859 staining human NR2F2 in convoluted tubule by Immunohistochemistry (FFPE-sections) 10ug/ml.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR2F2 antibody [H7147] (ab41859)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR2F2 antibody [H7147] (ab41859)
    ab41859 staining rat NR2F2 in Glomerular by Immunohistochemistry (FFPE-sections) 10ug/ml.
  • Western blot - Anti-NR2F2 antibody [H7147] (ab41859)
    Western blot - Anti-NR2F2 antibody [H7147] (ab41859)
    All lanes : Anti-NR2F2 antibody [H7147] (ab41859) at 1 µg/ml

    Lane 1 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 46 kDa
    Observed band size: 49 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 72 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 4 minutes

参考文献

This product has been referenced in:
  • Kim BJ & Scott DA Mouse model reveals the role of RERE in cerebellar foliation and the migration and maturation of Purkinje cells. PLoS One 9:e87518 (2014). IHC-P ; Mouse . Read more (PubMed: 24466353) »
  • Chan CM  et al. A signature motif mediating selective interactions of BCL11A with the NR2E/F subfamily of orphan nuclear receptors. Nucleic Acids Res N/A:N/A (2013). Read more (PubMed: 23975195) »

See all 4 Publications for this product

ab41859 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

レビューと Q&A

レビューを送る 質問を送る

4 reviews or Q&A

10
Votes: Highest First

Immunocytochemistry abreview for Anti-NR2F2 antibody [H7147]

 Average
Abreviews
Application
Immunocytochemistry
Sample
Mouse Cell (BRAIN)
Permeabilization
Yes - 4% paraform
Specification
BRAIN
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Fixative
Paraformaldehyde
Read More
abreview image
Username

Kyungjoo Seong

Verified customer

投稿 Jun 20 2017

Immunocytochemistry/ Immunofluorescence abreview for Anti-NR2F2 antibody [H7147]

 Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (human pluripotent stem cell derived cardiomyocytes)
Permeabilization
Yes - saponin
Specification
human pluripotent stem cell derived cardiomyocytes
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More
abreview image
Username

Abcam user community

Verified customer

投稿 Aug 20 2015

Dear xxxx,

Thank you for the answer. Yes, I would like to test ab41859 in ChIP experiments and would like to make a purchase of it. It would be grateful, if you could advise more on this, enroll us in the AbTrial testing program and issue me ...

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Thank you for your reply.
xxxx is currently away from the office but I will try to help with your request.
xxxx
I am very pleased to hear you would like to accept our offer and test ab41859 in ChIP. This code will give you €370 off your nex...

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Inquiry: I would like to know, if this product can be used for 'ChIP' experiments and also would like to order this antibody. kindly provide me with a quote. Thanks

Thank you for your enquiry.

I am sorry to confirm that as far as we are aware, ab41859 NR2F2 antibody [H7147] has never been tested in ChIP. All tested applications are specified on our datasheets, and these are updated as soon as any new in...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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