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Our Abpromise guarantee covers the use of ab41859 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use a concentration of 0.1 µg/ml.|
|IHC-P||Use a concentration of 10 µg/ml.|
|IP||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 46 kDa).|
Immnocytochemical immunoflurescence analysis of PFA-fixed human pluripotent stem cell derived cardiomyocytes, labelling NR2F2 with ab41859 at a concentration of 1/1000 incubated for 16 hours at 4°C. Permeablisation is using saponin. Blocking was done with 5% serum for 15 minutes at room temperature. Secondary used was a goat anti-mouse Alexa Fluor® 568 at 1/2000. The left panel shows the experimental conditions described formerly. The right panel is additionally counterstained with DAPI to show all cell nuclei within the area.