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Our Abpromise guarantee covers the use of ab50487 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-P||Use a concentration of 4 - 8 µg/ml.|
|ELISA||Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/312500.
Immunohistochemistry (Formalin/PFA-fixed paraffine-embedded sections) analysis of human kidney tissue labelling NRF2 with ab50487 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of renal tubule. Magnification: 400x.