製品の概要

  • 製品名Anti-NR2F2 antibody
    NR2F2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to NR2F2
  • アプリケーション適用あり: WB, IHC-P, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow, Dog, Pig, Zebrafish
  • 免疫原

    A region within synthetic peptide: KVGMRREAVQ RGRMPPTQPT HGQFALTNGD PLNCHSYLSG YISLLLRAEP, corresponding to amino acids 141-190 of Human NR2F2

  • ポジティブ・コントロール
    • Transfected 293T cell lysate; Epithelial cells of renal tubule of human kidney

アプリケーション

Our Abpromise guarantee covers the use of ab50487 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

ターゲット情報

Anti-NR2F2 antibody 画像



  • Predicted band size : 46 kDa
    Western blot analysis of MCF7 whole cell lysate labelling NR2F2 with ab50487 at 1.0µg/ml.
  • Lane 1 : Marker
    Lane 2 : Anti-NR2F2 antibody (ab50487) at 1 µg/ml

    Lane 1 : As above
    Lane 2 : Transfected 293T cell lysate

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 46 kDa
    Observed band size : 46 kDa
    Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffine-embedded sections) analysis of human kidney tissue labelling NRF2 with ab50487 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of renal tubule. Magnification: 400x.

Anti-NR2F2 antibody (ab50487) 使用論文

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Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (human pluripotent stem cell derived cardiomyocytes)
Specification human pluripotent stem cell derived cardiomyocytes
Blocking step Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative Paraformaldehyde
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投稿 Aug 20 2015

The immunogen sequence is 100% homologous between mouse and human species. This is a good indication that the antibody will recognize mouse NR2F2 protein. However the cross-reactivity is not guaranteed as we have not tested this.

Thank you for your enquiry.The immunogen sequence used to generate this antibody is located after amino acid 147 of human COUP-TF2. Please contact us if you have any questions.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"