製品の概要

  • 製品名Anti-NR2F2 antibody
    NR2F2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to NR2F2
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Rat, Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human NR2F2.

    (Peptide available as ab42826.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following lysates: HeLa Whole Cell Colon (Rat) Tissue Thymus (Rat) Tissue Spleen (Rat) Tissue Caco 2 Whole Cell HepG2 Whole Cell

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab42672 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/250. Detects a band of approximately 42 kDa (predicted molecular weight: 46 kDa).
IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 機能Ligand-activated transcription factor. Activated by high concentrations of 9-cis-retinoic acid and all-trans-retinoic acid, but not by dexamethasone, cortisol or progesterone (in vitro). Regulation of the apolipoprotein A-I gene transcription. Binds to DNA site A.
  • 組織特異性Ubiquitous.
  • 関連疾患Congenital heart defects, multiple types, 4
  • 配列類似性Belongs to the nuclear hormone receptor family. NR2 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Apolipoprotein A-I regulatory protein 1 antibody
    • Apolipoprotein AI regulatory protein 1 antibody
    • ARP-1 antibody
    • ARP1 antibody
    • COT2_HUMAN antibody
    • COUP TF2 antibody
    • COUP transcription factor 2 antibody
    • COUP transcription factor II antibody
    • COUP-TF II antibody
    • COUP-TF2 antibody
    • NR2F2 antibody
    • Nuclear receptor subfamily 2 group F member 2 antibody
    see all

Anti-NR2F2 antibody 画像

  • All lanes : Anti-NR2F2 antibody (ab42672) at 1/250 dilution

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Rat Colon Tissue Lysate
    Lane 3 : Rat Thymus Tissue Lysate
    Lane 4 : Spleen (Rat) Tissue Lysate - normal tissue (ab29393)
    Lane 5 : Caco 2 whole cell lysate (ab3950)
    Lane 6 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 46 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 78 kDa. We are unsure as to the identity of these extra bands.

Anti-NR2F2 antibody (ab42672) 使用論文

ab42672 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (human pluripotent stem cell derived cardiomyocytes)
Permeabilization Yes - saponin
Specification human pluripotent stem cell derived cardiomyocytes
Blocking step Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative Paraformaldehyde
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Abcam user community

Verified customer

投稿 Aug 20 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Kidney)
Specification Kidney
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako Target Retrieval Solution
Permeabilization No
Blocking step 5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
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Mr. Antibody Solutions

Verified customer

投稿 Apr 08 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"