製品の概要

  • 製品名Anti-NR2C2 antibody [EPR1773(2)]
    NR2C2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR1773(2)] to NR2C2
  • アプリケーション適用あり: WB, IPmore details
    適用なし: ICC or IHC-P
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human NR2C2 aa 500 to the C-terminus.

  • ポジティブ・コントロール
    • PC3 cell lysate, 293T cell lysate; Human testis tissue
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • バッファーpH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノモノクローナル
  • クローン名EPR1773(2)
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab109301 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 67 kDa (predicted molecular weight: 65 kDa).
IP 1/10 - 1/100.
  • 追加情報Is unsuitable for ICC or IHC-P.
  • ターゲット情報

    • 機能Orphan nuclear receptor that can act as a repressor or activator of transcription. An important repressor of nuclear recptor signaling pathways such as retinoic acid receptor, retinoid X, vitamin D3 receptor, thyroid hormone receptor and estrogen receptor pathways. May regulate gene expression during the late phase of spermatogenesis. Together with NR2C1, forms the core of the DRED (direct repeat erythroid-definitive) complex that represses embryonic and fetal globin transcription including that of GATA1. Binds to hormone response elements (HREs) consisting of two 5'-AGGTCA-3' half site direct repeat consensus sequences. Plays a fundamental role in early embryonic development and embryonic stem cells. Required for normal spermatogenesis and cerebellum development. Appears to be important for neurodevelopmentally regulated behavior (By similarity). Activates transcriptional activity of LHCG. Antagonist of PPARA-mediated transactivation.
    • 配列類似性Belongs to the nuclear hormone receptor family. NR2 subfamily.
      Contains 1 nuclear receptor DNA-binding domain.
    • 発生段階Transiently repressed during the meiotic phase of spermatogenesis.
    • 翻訳後修飾Phosphorylation on Ser-19 and Ser-68 is an important regulator of NR2C2-mediated transcriptional activity. Phosphorylation on these residues recruits the corepressor, NRIP1, leading to transcripional repression, whereas the nonphosphorylated form preferentially recruits the coactivator, PCAF.
    • 細胞内局在Nucleus.
    • Information by UniProt
    • 参照データベース
    • 製品の状態There are 2 isoforms produced by alternative splicing.
    • 別名
      • hTAK1 antibody
      • Nr2c2 antibody
      • NR2C2_HUMAN antibody
      • Nuclear hormone receptor TR4 antibody
      • Nuclear receptor subfamily 2 group C member 2 antibody
      • Orphan nuclear receptor TAK1 antibody
      • Orphan nuclear receptor TR4 antibody
      • TAK1 antibody
      • Testicular nuclear receptor 4 antibody
      • Testicular receptor 4 antibody
      • TR2R1 antibody
      • TR4 antibody
      • TR4 nuclear hormone receptor antibody
      see all

    Anti-NR2C2 antibody [EPR1773(2)] 画像

    • All lanes : Anti-NR2C2 antibody [EPR1773(2)] (ab109301) at 1/5000 dilution (purified)

      Lane 1 : PC-12 whole cell lysate
      Lane 2 : 3T3-L1 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 65 kDa
      Observed band size : 67 kDa (why is the actual band size different from the predicted?)

      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Anti-NR2C2 antibody [EPR1773(2)] (ab109301) at 1/5000 dilution (purified) + HEK293 whole cell lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 65 kDa
      Observed band size : 67 kDa (why is the actual band size different from the predicted?)

      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Anti-NR2C2 antibody [EPR1773(2)] (ab109301) at 1/2000 dilution (purified) + PC-3 whole cell lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 65 kDa
      Observed band size : 67 kDa (why is the actual band size different from the predicted?)
    • ab109301 (purified) at 1/30 immunoprecipitating NR2C2 in 10 μg HEK293 cell lysate (Lanes 1 and 2, observed at 67 kDa). Lane 3 - Rabbit monoclonal IgG (ab172730). For western blotting, HRP Veriblot for IP (ab131366) was used as the secondary antibody (1/10 000). Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST
    • All lanes : Anti-NR2C2 antibody [EPR1773(2)] (ab109301) at 1/1000 dilution (Unpurified)

      Lane 1 : PC3 cell lysate
      Lane 2 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 65 kDa
      Observed band size : 67 kDa (why is the actual band size different from the predicted?)

    Anti-NR2C2 antibody [EPR1773(2)] (ab109301) 使用論文

    ab109301 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab109301.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"