製品の概要

  • 製品名
    Anti-NR0B2 antibody
    NR0B2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to NR0B2
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee
  • 免疫原

    Synthetic peptide within Human NR0B2 aa 146-195 (internal sequence). The exact sequence is proprietary.
    Sequence:

    VQWLQCCLESFWSLELSPKEYACLKGTILFNPDVPGLQAASHIGHLQQEA


    Database link: Q15466

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • ポジティブ･コントロール
    • Transfected 293T cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab80417 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

ターゲット情報

  • 機能
    Acts as a transcriptional regulator. Acts as a negative regulator of receptor-dependent signaling pathways. Specifically inhibits transactivation of the nuclear receptor with whom it interacts. Inhibits transcriptional activity of NEUROD1 on E-box-containing promoter by interfering with the coactivation function of the p300/CBP-mediated trancription complex for NEUROD1.
  • 組織特異性
    Liver. Low levels of expression were detected in heart and pancreas.
  • 関連疾患
    Obesity
  • 配列類似性
    Belongs to the nuclear hormone receptor family. NR0 subfamily.
  • 翻訳後修飾
    Arginine methylation by PRMT5 enhances repression activity of metabolic genes in liver in response to bile acid signaling, by increasing interaction with cofactors.
  • 細胞内局在
    Nucleus. Cytoplasm. Colocalizes with NEUROD1 in the nucleus.
  • Information by UniProt
  • 参照データベース
    see all
  • 別名
    • NR0B2 antibody
    • NR0B2_HUMAN antibody
    • Nr0b2a antibody
    • Nuclear receptor subfamily 0 group B member 2 antibody
    • Nuclear receptor subfamily 0, group B, member 2a antibody
    • Orphan nuclear receptor SHP antibody
    • SHP antibody
    • SHP-1 antibody
    • Shp1 antibody
    • Small heterodimer partner antibody
    see all

画像

  • Western blot - NR0B2 antibody (ab80417)
    Western blot - NR0B2 antibody (ab80417)
    Anti-NR0B2 antibody (ab80417) at 1 µg/ml + Transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 29 kDa
    Observed band size: 29 kDa
    Additional bands at: 20 kDa, 39 kDa, 45 kDa. We are unsure as to the identity of these extra bands.



    Gel concentration: 12%

プロトコール

参考文献

ab80417 has not yet been referenced specifically in any publications.

ab80417 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

レビューと Q&A

レビューを送る 質問を送る

There are currently no Customer reviews or Questions for ab80417.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録