製品の概要

  • 製品名Anti-NR0B1 / Dax1 antibody
    NR0B1 / Dax1 一次抗体 製品一覧
  • 製品の詳細
    Mouse polyclonal to NR0B1 / Dax1
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Mouse
  • 免疫原

    Fusion protein:

    SLLTSAQQTHVSREAPEAHRRGEWWQLSYCTQSVGGPEGLQSTQAMAFLY RSYVCGEEQPQQISVASGTPVSADQTPATPQEQPRAPWWDASPGVQRLIT

    , corresponding to amino acids 150/249 of Mouse NR0B1.

  • 特記事項Produced from outbred CD1 mice


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab21884 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 52 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

ターゲット情報

  • 機能Orphan nuclear receptor. Component of a cascade required for the development of the hypothalamic-pituitary-adrenal-gonadal axis. Acts as a coregulatory protein that inhibits the transcriptional activity of other nuclear receptors through heterodimeric interactions. May also have a role in the development of the embryo and in the maintenance of embryonic stem cell pluripotency.
  • 関連疾患Defects in NR0B1 are the cause of X-linked adrenal hypoplasia congenital (XL-AHC) [MIM:300200]; also known as X-linked Addison disease (AHX). XL-AHC is a developmental disorder of the adrenal gland that results in profound hormonal deficiencies and is lethal if untreated. It is characterized by the absence of the permanent zone of the adrenal cortex and by a structural disorganization of the glands. Hypogonadotropic hypogonadism (HHG) is frequently associated with this disorder. HHG is a condition resulting from or characterized by abnormally decreased gonadal function, with retardation of growth and sexual development.
    Defects in NR0B1 are the cause of 46,XY sex reversal type 2 (SRXY2) [MIM:300018]. It is a condition characterized by male-to-female sex reversal in the presence of a normal 46,XY karyotype. Note=XY individuals with a duplication of part of the short arm of the X chromosome and an intact SRY gene develop as females. The single X chromosome in these individuals does not undergo X-chromosome inactivation; therefore, these individuals presumably carry 2 active copies of genes, including the NR0B1 gene, in the duplicated region. Individuals with deletion of this region develop as males. Genes within the dosage-sensitive sex reversal region are, therefore, not essential for testis development, but, when present in a double dose, interfere with testis formation.
  • 配列類似性Belongs to the nuclear hormone receptor family. NR0 subfamily.
  • ドメインHomodimerization involved an interaction between amino and carboxy termini involving LXXLL motifs and steroid binding domain (AF-2 motif). Heterodimerizes with NR5A1 and NROB2 through its N-terminal LXXLL motifs.
  • 細胞内局在Nucleus. Cytoplasm. Shuttles between the cytoplasm and nucleus. Homodimers exits in the cytoplasm and in the nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AHC antibody
    • AHCH antibody
    • AHX antibody
    • DAX 1 antibody
    • DAX1 antibody
    • Dosage sensitive sex reversal antibody
    • DSS antibody
    • DSS AHC critical region on the X chromosome protein 1 antibody
    • DSS-AHC critical region on the X chromosome protein 1 antibody
    • GTD antibody
    • HHG antibody
    • Nr0b1 antibody
    • NR0B1_HUMAN antibody
    • NROB1 antibody
    • Nuclear hormone receptor antibody
    • Nuclear receptor 0B1 antibody
    • Nuclear receptor DAX 1 antibody
    • Nuclear receptor DAX-1 antibody
    • Nuclear receptor DAX1 antibody
    • Nuclear receptor subfamily 0 group B member 1 antibody
    • SRXY2 antibody
    see all

Anti-NR0B1 / Dax1 antibody 画像

  • All lanes : Anti-NR0B1 / Dax1 antibody (ab21884) at 1/1000 dilution

    Lane 1 : Total protein extract from E. coli with ~50ng to 100ng of a negative control fusion protein with an irrelevant antigen at 20 ug
    Lane 2 : Total protein extract from E. coli with ~50ng to 500ng of the antigen fusion protein at 20 ug

    Secondary
    Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution

    Predicted band size : 52 kDa

Anti-NR0B1 / Dax1 antibody (ab21884) 使用論文

ab21884 has not yet been referenced specifically in any publications.

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