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Synthetic peptide contain a sequence corresponding to a region within amino acids 213 and 224 of Human NQO1 9(NP_000894).
Our Abpromise guarantee covers the use of ab97385 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 31 kDa. In some tested cell lines double bands (~26-34kDa) are seen: 293T, A431, H1299, HeLa, HepG2.|
|IHC-P||1/100 - 1/500.|
|ICC/IF||1/100 - 1/200.|
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human ovarian endometriosis tissue, staining NQO1 with ab97385.
Antigen retrieval was performed in sodium citrate buffer in a microwave oven. Samples were blocked with rabbit serum for one hour before incubating overnight with primary antibody (1/200). Stainined was detected using DAB.