製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Nptx1
  • アプリケーション
    適用あり: WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Rat, Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic peptide within Rat Nptx1 aa 174-203 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.

  • ポジティブ・コントロール
    • Jurkat cell line lysates, human breast carcinoma tissue and human brain tissue

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    Protein G purified
  • 特記事項(精製)
    This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab75917 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ELISA
IHC-P
  • 追加情報
    ELISA: 1/1000.
    IHC-P: 1/50 - 1/100.
    WB: 1/50 - 1/100. Detects bands of approximately 47 and 60 kDa (predicted molecular weight: 47 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    画像

    • Anti-Nptx1 antibody (ab75917) at 1/60 dilution + Jurkat cell line lysates at 35 µg

      Predicted band size : 47 kDa
      Observed band size : 47 kDa
      Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
    • Formalin-fixed and paraffin-embedded human breast carcinoma reacted with ab75917 at a 1/50 dilution, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
    • Formalin-fixed and paraffin-embedded human brain tissue reacted with ab75917 at a 1/50 dilution, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

    参考文献

    ab75917 has not yet been referenced specifically in any publications.

    レビューと Q&A

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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