製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to NPTN
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 108-157 (SAPKANATIE VKAAPDITGH KRSENKNEGQ DATMYCKSVG YPHPDWIWRK) of Human NPTN (NP_059429).

  • ポジティブ・コントロール
    • Fetal muscle lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab83063 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 32 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能
    Probable homophilic and heterophilic cell adhesion molecule involved in long term potentiation at hippocampal excitatory synapses through activation of p38MAPK. May also regulate neurite outgrowth by activating the FGFR1 signaling pathway. May play a role in synaptic plasticity.
  • 組織特異性
    Isoform 1 is ubiquitously expressed. Isoform 2 is expressed in brain cortex and cerebellum (at protein level).
  • 配列類似性
    Contains 3 Ig-like (immunoglobulin-like) domains.
  • ドメイン
    Some isoforms lack the first Ig-like domain which may confer homophilic adhesion activity. However, they can bind and activate FGFR1.
  • 翻訳後修飾
    N-glycosylated.
  • 細胞内局在
    Cell membrane. Enriched at post-synaptic density.
  • Information by UniProt
  • 参照データベース
  • 別名
    • GP55 antibody
    • GP65 antibody
    • Neuroplastin antibody
    • np55 antibody
    • np65 antibody
    • Nptn antibody
    • NPTN_HUMAN antibody
    • SDFR1 antibody
    • SDR-1 antibody
    • SDR1 antibody
    • stromal cell derived factor receptor 1 antibody
    • Stromal cell-derived receptor 1 antibody
    see all

Anti-NPTN antibody 画像

  • Anti-NPTN antibody (ab83063) at 1 µg/ml (5% skim milk / PBS buffer) + Fetal muscle lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 32 kDa
    Observed band size : 31 kDa (why is the actual band size different from the predicted?)

プロトコール

Anti-NPTN antibody (ab83063) 使用論文

ab83063 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us about the issues you are having with the FGFR1 and NPTN antibodies. Can you please send me a few details of your samples and the protocols you use for harvesting the samples and for blotting? In particular, I am interested i...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain Tissue)
Loading amount
40 µg
Specification
Brain Tissue
Gel Running Conditions
Non-reduced Denaturing (10% Bis-Tris)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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投稿 May 27 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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