製品の概要

  • 製品名
  • 製品の詳細
    Mouse polyclonal to NPR2L
  • 由来種
    Mouse
  • アプリケーション
    適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length human NPR2L protein (AAH21984.1).

  • ポジティブ・コントロール
    • WB: HeLa cell lysate; NPR2L transfected 293T cell lysate ICc/IF: HeLa cell

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab88691 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 44 kDa.
ICC/IF Use a concentration of 10 µg/ml.

ターゲット情報

  • 機能
    Suppresses Src-dependent tyrosine phosphorylation and activation of PDPK1 and its downstream signaling. Down-regulates PDPK1 kinase activity by interfering with tyrosine phosphorylation at the Tyr-9 Tyr-373 and Tyr-376 residues. May act as a tumor suppressor. Suppresses cell growth and enhanced sensitivity to various anticancer drugs.
  • 組織特異性
    Most abundant in skeletal muscle, followed by brain, liver and pancreas, with lower amounts in lung, kidney, placenta and heart. Expressed in most lung cancer cell lines tested.
  • 配列類似性
    Belongs to the NPR2 family.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 2810446G01Rik antibody
    • G21 protein antibody
    • Gene 21 protein antibody
    • Homologous to yeast nitrogen permease (candidate tumor suppressor) antibody
    • Homologous to yeast nitrogen permease antibody
    • Nitrogen permease regulator 2-like protein antibody
    • Nitrogen permease regulator-like 2 (S. cerevisiae) antibody
    • NPR 2L antibody
    • NPR L2 antibody
    • NPR like 2 antibody
    • NPR2 antibody
    • NPR2 like antibody
    • NPR2-like protein antibody
    • NPR2L antibody
    • NPRL 2 antibody
    • NPRL2 antibody
    • NPRL2_HUMAN antibody
    • Tumor suppressor candidate 4 antibody
    • TUSC 4 antibody
    • TUSC 4 protein antibody
    • TUSC4 antibody
    • TUSC4 protein antibody
    see all

画像

  • Anti-NPR2L antibody (ab88691) at 1 µg/ml + HeLa cell lysate at 50 µg

    Predicted band size: 44 kDa
    Observed band size: 44 kDa

  • All lanes : Anti-NPR2L antibody (ab88691) at 1 µg/ml

    Lane 1 : NPR2L transfected 293T cell lysate
    Lane 2 : non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Predicted band size: 44 kDa
    Observed band size: ~37 kDa (why is the actual band size different from the predicted?)

  • ab88691, at 10 µg/ml, staining NPR2L in HeLa cell by Immunofluorescence.

参考文献

This product has been referenced in:
  • Liu S & Liu B Overexpression of Nitrogen Permease Regulator Like-2 (NPRL2) Enhances Sensitivity to Irinotecan (CPT-11) in Colon Cancer Cells by Activating the DNA Damage Checkpoint Pathway. Med Sci Monit 24:1424-1433 (2018). Read more (PubMed: 29519997) »
  • Liu AY  et al. Relationship between tumor and peripheral blood NPRL2 mRNA levels in patients with colorectal adenoma and colorectal cancer. Cancer Biol Ther 15:489-95 (2014). Read more (PubMed: 24521741) »

See all 2 Publications for this product

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