製品の概要

  • 製品名Anti-NPL4 antibody
    NPL4 一次抗体 製品一覧
  • 製品の詳細
    Mouse polyclonal to NPL4
  • 特異性This antibody is reactive against mammalian transfected lysate.
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    NPLOC4 (AAH25930.1, 1 a.a. ~ 608 a.a) full-length human protein.

  • ポジティブ・コントロール
    • Transfected 293T cell lysate and human spleen tissue lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab68020 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
  • 追加情報WB: 1/500 - 1/1000. Detects bands of approximately 68 and 75 kDa (predicted molecular weight: 68 kDa).


    ir.Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope.
    • 組織特異性Expressed at highest levels in brain, heart, skeletal muscle, kidney and fetal liver.
    • パスウェイProtein degradation; proteasomal ubiquitin-dependent pathway.
    • 配列類似性Belongs to the NPL4 family.
      Contains 1 RanBP2-type zinc finger.
    • ドメインBinds ubiquitinated proteins via its RanBP2-type zinc finger.
    • 細胞内局在Cytoplasm > cytosol. Endoplasmic reticulum. Nucleus. Associated with the endoplasmic reticulum and nuclear.
    • Information by UniProt
    • 参照データベース
    • 別名
      • KIAA1499 antibody
      • NPL4 antibody
      • NPL4, S. cerevisiae, homolog of antibody
      • NPL4_HUMAN antibody
      • nploc4 antibody
      • nuclear protein localization 4 homolog (S. cerevisiae) antibody
      • Nuclear protein localization 4 homolog antibody
      • Nuclear protein localization protein 4 homolog antibody
      • Nuclear protein localization protein 4, S. cerevisiae, homolog of antibody
      • Protein NPL4 antibody
      see all

    Anti-NPL4 antibody 画像

    • Anti-NPL4 antibody (ab68020) at 1/500 dilution + human spleen tissue lysate at 25 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 68 kDa
      Observed band size : 75 kDa (why is the actual band size different from the predicted?)
    • All lanes : Anti-NPL4 antibody (ab68020) at 1/500 dilution

      Lane 1 : NPLOC4 transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 68 kDa
      Observed band size : 68 kDa

    Anti-NPL4 antibody (ab68020) 使用論文

    ab68020 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab68020.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"