The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Western Blot: use at 1:500-1:1,000 dilution.
ELISA: use at 1:1,000-1:5,000 dilution
Receptor for NPAF (A-18-F-amide) and NPFF (F-8-F-amide) neuropeptides, also known as morphine-modulating peptides. Can also be activated by a variety of naturally occurring or synthetic FMRF-amide like ligands. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
Belongs to the G-protein coupled receptor 1 family.