Anti-NPAT 抗体 (ab99436)
Key features and details
- Rabbit polyclonal to NPAT
- Suitable for: IHC-P, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-NPAT antibody
NPAT 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to NPAT -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee, Gorilla, Orangutan -
免疫原
Synthetic peptide corresponding to Human NPAT aa 700-800.
Database link: Q14207 -
ポジティブ・コントロール
- Human 293T cell line.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
-
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab99436の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P |
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
IP |
Use at 2-5 µg/mg of lysate.
|
特記事項 |
---|
IHC-P
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
-
関連性
NPAT is a 240kDa protein that associates with and can be phosphorylated by the CDK cyclin E–CDK2. The protein level of NPAT peaks at the G1/S boundary. Overexpression of NPAT accelerates S-phase entry suggesting that NPAT plays a role in S-phase entry. -
細胞内局在
Nuclear -
参照データベース
- Entrez Gene: 4863 Human
- Omim: 601448 Human
- SwissProt: Q13632 Human
- SwissProt: Q14207 Human
- SwissProt: Q16580 Human
-
別名
- E14 antibody
- nuclear protein ataxia telangiectasia locus antibody
- nuclear protein in the AT region antibody
画像
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling NPAT with ab99436 at 1/1000 (1µg/ml). Detection: DAB.
-
Immunoprecipitation : 1 mg of 293T cell lysate immunoprecipitated with ab99436 at 3 µg/mg lysate; 20% of immunoprecipitate loaded in lane
Western Blot: an antibody that recognizes a downstream epitope of NPAT at 1 µg/ml
developed using the ECL technique
Exposure time : 30 seconds
Predicted band size : 154 kDa
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab99436 は論文での使用が確認できていません。