Anti-NPAT 抗体 [2648C6a] (ab70595)
Key features and details
- Mouse monoclonal [2648C6a] to NPAT
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG1
製品の概要
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製品名
Anti-NPAT antibody [2648C6a]
NPAT 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [2648C6a] to NPAT -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Recombinant fragment -
免疫原
Recombinant fragment (Human) from an internal sequence of NPAT.
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ポジティブ・コントロール
- Recombinant human NPAT.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.1136% Dibasic monohydrogen sodium phosphate, 1% BSA, 0.03% Potassium phosphate, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.0225% Potassium chloride, 0.0204% Monobasic dihydrogen potassium phosphate -
Concentration information loading...
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精製度
Protein G purified -
特記事項(精製)
This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22 µm membrane. -
ポリ/モノ
モノクローナル -
クローン名
2648C6a -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70595の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 240 kDa.
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特記事項 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 240 kDa. |
ターゲット情報
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関連性
NPAT is a 240kDa protein that associates with and can be phosphorylated by the CDK cyclin E–CDK2. The protein level of NPAT peaks at the G1/S boundary. Overexpression of NPAT accelerates S-phase entry suggesting that NPAT plays a role in S-phase entry. -
細胞内局在
Nuclear -
別名
- E14 antibody
- nuclear protein ataxia telangiectasia locus antibody
- nuclear protein in the AT region antibody
画像
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Anti-NPAT antibody [2648C6a] (ab70595) + immunising recombinant fragment
Predicted band size: 240 kDa
Observed band size: 44 kDa why is the actual band size different from the predicted?
The molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the protein was used.
データシートおよび資料
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Datasheet download
参考文献 (4)
ab70595 は 4 報の論文で使用されています。
- Quinodoz SA et al. RNA promotes the formation of spatial compartments in the nucleus. Cell 184:5775-5790.e30 (2021). PubMed: 34739832
- Qian W et al. Secretome analysis of rat osteoblasts during icariin treatment induced osteogenesis. Mol Med Rep 17:6515-6525 (2018). PubMed: 29532868
- Huang X & Lyu J Tumor suppressor function of miR-483-3p on breast cancer via targeting of the cyclin E1 gene. Exp Ther Med 16:2615-2620 (2018). PubMed: 30186493
- Mano M et al. Genome-wide RNAi screening identifies host restriction factors critical for in vivo AAV transduction. Proc Natl Acad Sci U S A 112:11276-81 (2015). PubMed: 26305933