製品の概要

  • 製品名
    Anti-NPAT antibody [2648C6a]
    NPAT 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [2648C6a] to NPAT
  • 由来種
    Mouse
  • アプリケーション
    適用あり: WB, Dot blotmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment (Human) from an internal sequence of NPAT.

  • ポジティブ・コントロール
    • Recombinant human NPAT.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    Protein G purified
  • 特記事項(精製)
    This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22 µm membrane.
  • ポリ/モノ
    モノクローナル
  • クローン名
    2648C6a
  • アイソタイプ
    IgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab70595 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent dilution. Predicted molecular weight: 240 kDa.
Dot blot Use at an assay dependent dilution.

ターゲット情報

  • 関連性
    NPAT is a 240kDa protein that associates with and can be phosphorylated by the CDK cyclin E–CDK2. The protein level of NPAT peaks at the G1/S boundary. Overexpression of NPAT accelerates S-phase entry suggesting that NPAT plays a role in S-phase entry.
  • 細胞内局在
    Nuclear
  • 参照データベース
  • 別名
    • E14 antibody
    • nuclear protein ataxia telangiectasia locus antibody
    • nuclear protein in the AT region antibody

画像

  • Anti-NPAT antibody [2648C6a] (ab70595) + immunising recombinant fragment

    Predicted band size: 240 kDa
    Observed band size: 44 kDa (why is the actual band size different from the predicted?)



    The molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the protein was used.

プロトコール

参考文献

This product has been referenced in:
  • Qian W  et al. Secretome analysis of rat osteoblasts during icariin treatment induced osteogenesis. Mol Med Rep 17:6515-6525 (2018). Read more (PubMed: 29532868) »
  • Mano M  et al. Genome-wide RNAi screening identifies host restriction factors critical for in vivo AAV transduction. Proc Natl Acad Sci U S A 112:11276-81 (2015). Read more (PubMed: 26305933) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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