Anti-NOXA2/p67phox 抗体 [EPR5065] (ab109523)


  • 製品名Anti-NOXA2/p67phox antibody [EPR5065]
    NOXA2/p67phox 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR5065] to NOXA2/p67phox
  • アプリケーション適用あり: WB, IHC-P, ICC, Flow Cytmore details
    適用なし: IP
  • 種交差性
    交差種: Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human NOXA2/p67phox

  • ポジティブ・コントロール
    • THP-1 or HL-60 treated with Dexomethasone and TPA cell lysate Human colon tissue
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.




Our Abpromise guarantee covers the use of ab109523 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/10000 - 1/50000. Detects a band of approximately 67 kDa (predicted molecular weight: 60 kDa).
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC 1/500 - 1/1000.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • 追加情報Is unsuitable for IP.
  • ターゲット情報

    • 機能NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).
    • 関連疾患Defects in NCF2 are a cause of chronic granulomatous disease autosomal recessive cytochrome-b-positive type 2 (CGD2) [MIM:233710]. Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections.
    • 配列類似性Belongs to the NCF2/NOXA1 family.
      Contains 1 OPR domain.
      Contains 2 SH3 domains.
      Contains 3 TPR repeats.
    • 細胞内局在Cytoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 67 kDa neutrophil oxidase factor antibody
      • Chronic granulomatous disease autosomal 2 antibody
      • FLJ93058 antibody
      • NADPH oxidase activator 2 antibody
      • NCF-2 antibody
      • Ncf2 antibody
      • NCF2_HUMAN antibody
      • Neutrophil cytosol factor 2 antibody
      • Neutrophil cytosolic factor 2 (65kD, chronic granulomatous disease, autosomal 2) antibody
      • Neutrophil NADPH oxidase factor 2 antibody
      • NOXA2 antibody
      • P67 PHOX antibody
      • p67-phox antibody
      • p67phox antibody
      see all

    Anti-NOXA2/p67phox antibody [EPR5065] 画像

    • All lanes : Anti-NOXA2/p67phox antibody [EPR5065] (ab109523) at 1/10000 dilution

      Lane 1 : THP-1 cell lysate
      Lane 2 : HL-60 treated with Dexomethasone and TPA cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size : 60 kDa
      Observed band size : 67 kDa (why is the actual band size different from the predicted?)
    • ab109523, at a 1/500 dilution, staining Human NOXA2/p67phox in colon, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.
    • Flow cytometry analysis of permeabilized THP-1 cells using ab109523 (red) and a rabbit IgG (negative contol) (green). The dilution is 1:150. The secondary antibody is a goat anti-rabbit IgG FITC.

    Anti-NOXA2/p67phox antibody [EPR5065] (ab109523) 使用論文

    ab109523 has not yet been referenced specifically in any publications.

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