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RabMAb

Anti-NOXA2/p67phox 抗体 [EPR5065] (ab109523)

製品の概要

  • 製品名
    Anti-NOXA2/p67phox antibody [EPR5065]
    NOXA2/p67phox 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR5065] to NOXA2/p67phox
  • アプリケーション
    適用あり: WB, IHC-P, ICC, Flow Cytmore details
    適用なし: IP
  • 種交差性
    交差種: Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human NOXA2/p67phox

  • ポジティブ・コントロール
    • THP-1 or HL-60 treated with Dexomethasone and TPA cell lysate Human colon tissue
  • 特記事項

     

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab109523 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/10000 - 1/50000. Detects a band of approximately 67 kDa (predicted molecular weight: 60 kDa).
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC 1/500 - 1/1000.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • 追加情報
    Is unsuitable for IP.
  • ターゲット情報

    • 機能
      NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).
    • 関連疾患
      Defects in NCF2 are a cause of chronic granulomatous disease autosomal recessive cytochrome-b-positive type 2 (CGD2) [MIM:233710]. Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections.
    • 配列類似性
      Belongs to the NCF2/NOXA1 family.
      Contains 1 OPR domain.
      Contains 2 SH3 domains.
      Contains 3 TPR repeats.
    • 細胞内局在
      Cytoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 67 kDa neutrophil oxidase factor antibody
      • Chronic granulomatous disease autosomal 2 antibody
      • FLJ93058 antibody
      • NADPH oxidase activator 2 antibody
      • NCF-2 antibody
      • Ncf2 antibody
      • NCF2_HUMAN antibody
      • Neutrophil cytosol factor 2 antibody
      • Neutrophil cytosolic factor 2 (65kD, chronic granulomatous disease, autosomal 2) antibody
      • Neutrophil NADPH oxidase factor 2 antibody
      • NOXA2 antibody
      • P67 PHOX antibody
      • p67-phox antibody
      • p67phox antibody
      see all

    画像

    • All lanes : Anti-NOXA2/p67phox antibody [EPR5065] (ab109523) at 1/10000 dilution

      Lane 1 : THP-1 cell lysate
      Lane 2 : HL-60 treated with Dexomethasone and TPA cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 60 kDa
      Observed band size : 67 kDa (why is the actual band size different from the predicted?)
    • ab109523, at a 1/500 dilution, staining Human NOXA2/p67phox in colon, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.
    • Flow cytometry analysis of permeabilized THP-1 cells using ab109523 (red) and a rabbit IgG (negative contol) (green). The dilution is 1:150. The secondary antibody is a goat anti-rabbit IgG FITC.

    参考文献

    This product has been referenced in:
    • Hayashi G & Cortopassi G Lymphoblast Oxidative Stress Genes as Potential Biomarkers of Disease Severity and Drug Effect in Friedreich's Ataxia. PLoS One 11:e0153574 (2016). WB . Read more (PubMed: 27078885) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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